砀山酥梨炭疽病病原鉴定和检测及致病性分析  

Identification,detection and pathogenicity analysis of‘Dangshan Su’pear anthracnose

作　　者：周贝贝 高正辉[2,4,5] 杨雪 石旺鹏 齐永杰 马娜[2,4,5] 阚丽平 徐义流 ZHOU Beibei;GAO Zhenghui;YANG Xue;SHI Wangpeng;QI Yongjie;MA Na;KAN Liping;XU Yiliu(College of Plant Protection,China Agricultural University,Beijing 100193,China;Institute of Horticulture,Anhui Academy of Agricultural Sciences,Hefei 230001,China;Institute of Plant Protection and Agro-products Safety,Anhui Academy of Agricultural Sciences,Hefei 230001,China;Key Laboratory of Horticultural Crop Genetic Improvement and Eco-physiology of Anhui Province,Hefei 230001,China;Key Laboratory of Horticultural Crop Germplasm Innovation and Utilization(Co-construction by Ministry and Province),Institute of Horticulture,Anhui Academy of Agricultural Sciences,Hefei 230001,China)

机构地区：[1]中国农业大学植物保护学院,北京100193 [2]安徽省农业科学院园艺研究所,合肥230001 [3]安徽省农业科学院植物保护与农产品质量安全研究所,合肥230001 [4]园艺作物种质创制及生理生态安徽省重点实验室,合肥230001 [5]农业农村部园艺作物种质创制与利用重点实验室(部省共建),合肥230001

出　　处：《植物病理学报》2024年第3期648-653,共6页Acta Phytopathologica Sinica

基　　金：农业农村部园艺作物种质创制与利用重点实验室(部省共建)(2022年8月24日);园艺作物种质创制及生理生态安徽省重点实验室;国家梨产业技术体系专项资金资助(CARS-28);国家重点研发计划研究任务“安徽地区梨优质高效品种筛选与配套栽培技术(2019YFD1001404-1-3)”;安徽省农业科学院人才项目(LJRC-202108,QNYC-202112);安徽省农业科学院平台项目(2023YL015)。

摘　　要：0引言。梨炭疽病亦称苦腐病、晚腐病,自2008年在黄河故道梨产区爆发以来普遍发生,近年受气候变化、菌源累积、管理方式等因素影响,已成为安徽省砀山县及周边梨产区的主要病害之一。此病主要危害果实引起果腐,严重影响梨的产量和品质,给砀山县梨产业造成了极大的经济损失[1]。In recent years,pear anthracnose has become one of the major pear diseases in Dangshan County of Anhui Province and its surrounding areas.In order to identify the pathogens of‘Dangshan Su’pear anthracnose,the diseased samples were collected from Dangshan County and the pathogens were isolated,purified,and identified through morphology and molecular biology analysis.In this study,the pathogenicity of the pathogens was assayed by using the fruits and leaves of‘Dangshan Su’pear as materials.All these confirmed that the pathogens were mainly Colletotrichum fructicola and C.siamense.The specific detection primers Cf-F/Cf-R were designed according to part of the mRNA sequence of C.fructicola,which could specifically detect C.fructicola from similar strains and diseased tissues.

关 键 词：梨产区 病原鉴定 黄河故道 果腐 晚腐病 苦腐病 菌源 梨炭疽病 

分 类 号：S436.612[农业科学—农业昆虫与害虫防治]