院内制剂(协方)骨痹合剂纯化工艺研究  

Study on Purification Technology of In-Hospital Preparation (Co-Prescription) Gubi Mixture

作　　者：李佳 韩子璇 杨立茹 刘梦 包子威 张纯刚 Li Jia;Han Zixuan;Yang Liru;Liu Meng;Bao Ziwei;Zhang Chungang(College of Pharmacy,Liaoning University of Traditional Chinese Medicine,Dalian 116620,China;Department of Pharmacy,Changzhi Medical College,Changzhi 046000,China;Qimeng Co.,Ltd.,Chifeng 024330,China)

机构地区：[1]辽宁中医药大学药学院,辽宁大连116620 [2]长治医学院药学系,山西长治046000 [3]祈蒙股份有限公司,内蒙古赤峰024330

出　　处：《亚太传统医药》2024年第4期58-62,共5页Asia-Pacific Traditional Medicine

基　　金：内蒙古自治区科技重大专项(2021ZD0017);国家重点研发计划(2018YFC1706903);沈阳市中青年科技创新人才项目(RC200204)。

摘　　要：目的:优选骨痹合剂的纯化工艺。方法:以纯化后出膏率以及有效成分青藤碱、芍药苷、β-蜕皮甾酮的转移率为指标,分别考察碱沉淀法、平板膜超滤法和醇沉法对骨痹合剂原复方的纯化效果,以筛选最优的纯化方法。结果:在3种纯化工艺中,醇沉法纯化效果最好,当提取液浓缩至含生药量为1.9 g/mL,含醇量为80%,加醇体积为浓缩液体积4倍量时,出膏率可减少至10.28%,青藤碱、芍药苷、β-蜕皮甾酮3种有效成分的转移率分别为94.21%、92.86%、90.80%。结论:采用醇沉法对骨痹合剂纯化效果最好,该法操作简单、工艺稳定,可为制剂进一步开发提供参考。Objective:To optimize the purification technology of Gubi Mixture.Methods:Taking the paste yield and the transfer rate of the active ingredients sinomenine,paeoniflorin and β-ecdysterone as indexes,the purification effect of alkali precipitation method,plate membrane ultrafiltration method and alcohol precipitation method on Gubi mixture original compound were investigated respectively in order to screen the best purification method.Results:Alcohol precipitation method has the best purification effect among the three purification processes,When the decoction is concentrated to 1.9 μg/mL of raw herbs content,80% of alcohol content,and the volume of alcohol added is four times the volume of concentrated liquid,the paste yield was 10.28%,And the transfer rates of sinomenine,paeoniflorin and β-ecdysterone were 94.21%、92.86% and 90.80%.Conclusion:Gubi mixture has the best purification effect by alcohol sedimentation method,This method is simple and stable,which can provide a reference for the next development of drug preparation.

关 键 词：骨痹合剂 膝骨性关节炎 纯化工艺 出膏率 青藤碱 芍药苷 β-蜕皮甾酮 

分 类 号：R283[医药卫生—中药学]