角果木内生真菌Penicillium herquei JX4中2个新的pinophol类化合物  

Two new pinophol derivatives from endophytic fungus Penicillium herquei JX4 hosted in Ceriops tagal

作　　者：劳先磊 吴美珠 郑婷 陈光英[1] 郑彩娟[1] 陈雅林 周金琪 冯硕 周学明[1] 宋鑫明[1] LAO Xian-lei;WU Mei-zhu;ZHENG Ting;CHEN Guang-ying;ZHENG Cai-juan;CHEN Ya-lin;ZHOU Jin-qi;FENG Shuo;ZHOU Xue-ming;SONG Xin-ming(Key Laboratory of Tropical Medicinal Resource Chemistry of Ministry of Education,Key Laboratory of Tropical Medicinal Plant Chemistry of Hainan Province,Hainan Normal University,Haikou 571158,China)

机构地区：[1]海南师范大学热带药用资源化学教育部重点实验室、海南省热带药用植物化学重点实验室,海南海口571158

出　　处：《中国中药杂志》2024年第13期3548-3551,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(82260831);海南省“南海新星”科技创新人才项目(NHXXRCXM202315)。

摘　　要：应用OSMAC策略,对来源于红树角果木的内生真菌Penicillium herquei JX4进行次级代谢产物及其抗炎活性研究。综合运用硅胶柱色谱、凝胶柱色谱、ODS柱色谱以及半制备高效液相色谱技术对真菌P.herquei JX4的PDB发酵物进行分离纯化以及鉴定,共分离鉴定了2个pinophol类新化合物:pinophol H(1)和pinophol I(2)。对这2个化合物进行了脂多糖(LPS)诱导RAW264.7细胞NO生成抑制活性测试,结果表明化合物1具有显著的NO生产抑制活性,其IC_(50)为8.12μmol·L^(-1)。An OSMAC strategy was used to study secondary metabolites and anti-inflammatory activities of the endophytic fungus Penicillium herquei JX4 hosted in Ceriops tagal.The PDB ferment of fungus P.herquei JX4 was isolated,purified,and identified by using silica gel column chromatography,gel column chromatography,octadecylsilyl(ODS)column chromatography,and semi-preparative high-performance liquid chromatography.Two new pinophol derivatives,pinophol H(1)and pinophol I(2)were isolated and identified,and they were evaluated in terms of the inhibitory activities against the nitric oxide(NO)production induced by lipopolysaccharide(LPS)in mouse macrophage RAW264.7 cells.The results showed that compound 1 had significant inhibitory activity on NO production,with an IC_(50)value of 8.12μmol·L^(-1).

关 键 词：角果木 内生真菌 Penicillium herquei JX4 pinophol 抗炎 

分 类 号：R284.1[医药卫生—中药学]