UPLC指纹图谱结合一测多评法的芦蒿废弃茎叶酚酸类成分质量控制方法研究  

Quality control method for phenolic acid components in abandoned stems and leaves of Artemisia selengensis based on UPLC fingerprint combined with quantitative analysis of multi-components by single marker(QAMS)

作　　者：张国鑫 刘佳琪 恽辰珂 郭盛[1] 朱永强[2] 张森[1] ZHANG Guo-xin;LIU Jia-qi;YUN Chen-ke;GUO Sheng;ZHU Yong-qiang;ZHANG Sen(Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,Nanjing University of Chinese Medicine,Nanjing 210023,China;Department of Bioengineering,Suqian University,Suqian 223800,China)

机构地区：[1]南京中医药大学江苏省中药资源产业化过程协同创新中心,江苏南京210023 [2]宿迁学院生物工程系,江苏宿迁223800

出　　处：《中国中药杂志》2024年第13期3566-3573,共8页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金青年基金项目(221006073);江苏科技智库计划项目(JSKX24075);宿迁市科技计划指令性项目(K202232)。

摘　　要：该研究建立了芦蒿废弃茎叶的UPLC指纹图谱及5个酚酸类成分的一测多评法(quantitative analysis of multi-components by single marker,QAMS)。采用Waters Acquity UPLC BEH C_(18)色谱柱(2.1 mm×100 mm,1.7μm),流动相为0.1%磷酸水-乙腈,梯度洗脱,流速0.3 mL·min^(-1),检测波长330 nm,柱温30℃,进样量2μL。对指纹图谱的数据进行相似度评价及聚类分析,确定13批次芦蒿废弃茎叶中的15个共有成分。以绿原酸为内参物,分别计算阿魏酸、异绿原酸A、异绿原酸B、异绿原酸C的相对校正因子,实现了芦蒿废弃茎叶中5个成分含量测定的一测多评法的建立。同时采用外标法测定此5个成分的含量,结果显示一测多评法与外标法测定结果无显著性差异。结果表明,不同品种和不同产地的芦蒿废弃茎叶中的酚酸类成分含量存在明显差异,且木质化后的芦蒿废弃物中的酚酸类含量较未木质化显著提升。综上所述,该研究建立的芦蒿废弃茎叶一测多评法能够快速准确、简便经济地用于芦蒿废弃茎叶中5个酚酸成分的含量测定,为芦蒿废弃茎叶的资源化开发利用奠定了基础。This study established an ultra-performance liquid chromatography(UPLC)fingerprint of abandoned stems and leaves of Artemisia selengensis and quantitative analysis of multi-components by single marker(QAMS)for five phenolic acid components.Waters Acquity UPLC BEH C_(18)chromatography column(2.1 mm×100 mm,1.7μm)was used.The gradient elution was carried out with the mobile phase composed of 0.1%phosphoric acid water and acetonitrile at a flow rate of 0.3 mL·min^(-1)and a column temperature at 30℃.The detection wavelength was 330 nm,and the injection volume was 2μL.Similarity evaluation and cluster analysis were conducted on the fingerprint data,and 15 common components in 13 batches of abandoned stems and leaves of A.selengensis were identified.The relative correction factors of ferulic acid,isochlorogenic acid A,isochlorogenic acid B,and isochlorogenic acid C were calculated using chlorogenic acid as the internal reference.The QAMS for determining five components in the abandoned stems and leaves of A.selengensis was established.At the same time,the content of these five components was determined using the external standard method(ESM),and the results showed that there were no significant differences in their content determined by the QAMS and the ESM.The results indicated that the content of phenolic acid components in the abandoned stems and leaves of A.selengensis from different varieties and different origins had obvious differences.In addition,the content of phenolic acid components in the abandoned stems and leaves of lignified A.selengensis was significantly higher than that of non-lignified A.selengensis.In summary,QAMS established in this study can be quickly,accurately,and economically used to determine the content of five phenolic acid components in abandoned stems and leaves of A.selengensis,laying a foundation for the resource development and utilization of abandoned stems and leaves of A.selengensis.

关 键 词：芦蒿废弃茎叶 酚酸 一测多评法(QAMS) 外标法(ESM) 资源开发 

分 类 号：R284.1[医药卫生—中药学]