子宫内膜息肉组织中TLR4/NF-κB通路功能改变的研究  

To explore the value of TLR4/NF-κB pathway molecules and its downstream inflammatory factors in endometrial polyp tissue

作　　者：王意[1] 李子珊[2] 何春银 夏建红[1] WANG Yi;LI Zishan;HE Chunyin;XIA Jianhong(Department of Women's Health Care,Guangdong Women and Children Hospital,Guangzhou,Guangdong 511400,China;Department of Physical Examination,Guangdong Women and Children Hospital,Guangzhou,Guangdong 511400,China)

机构地区：[1]广东省妇幼保健院妇女保健科,广东广州511400 [2]广东省妇幼保健院体检科,广东广州511400

出　　处：《中国优生与遗传杂志》2024年第5期917-920,共4页Chinese Journal of Birth Health & Heredity

基　　金：广东省中医药局科研项目基金(20221043)。

摘　　要：目的研究子宫内膜息肉(EP)组织中TLR4/NF-κB通路分子与炎性因子的改变,探讨EP发生与子宫内膜局部炎症反应的相关性。方法选择在广东省妇幼保健院就诊的子宫内膜息肉患者作为EP组,同期因不孕症行宫腔镜检查且经病理证实为正常增生期子宫内膜的患者作为对照组。宫腔镜下冷刀切除术准确获得息肉组织标本,采用RT-qPCR和免疫组化法测定EP组息肉组织及对照组正常子宫内膜组织中TLR4/NF-κB通路分子、炎性因子白细胞介素(IL)-6的表达差异。结果EP组息肉组织中TLR4、NF-κB、IL-6的mRNA及蛋白质表达水平明显高于对照组正常子宫内膜组织。EP组息肉组织中增高的IL-6的表达与TLR4/NF-κB的表达呈正相关。结论EP组织TLR4/NF-κB通路的过度激活与炎性细胞因子IL-6分泌异常相关。提示子宫内膜息肉局部活跃的炎症反应及免疫失衡可能与子宫内膜息肉的发生发展相关。Objective To study the expression of TLR4(Toll-like receptor 4)/NF-κB(nuclear factorκB)pathway molecules and its downstream inflammatory factors in endometrial polyp(EP)tissue and to analyze the correlation with EP occurrence.Methods The patients with EP in Guangdong Women and Children Hospital were selected as the EP group,and the patients who underwent hysteroscopy due to infertility and were confirmed to be normal hyperplasia of endometrium by pathology were selected as the control group.Polyp tissue samples were accurately obtained by hysteroscopic cold knife resection.The expression of TLR4/NF-κB pathway molecules and its downstream inflammatory factors were measured.Results The mRNA and protein expression levels of TLR4,NF-κB and IL-6 in polyp tissue of EP groups were significantly higher than those in normal endometrial tissue of control groups.The mRNA expression of IL-6 in polyp tissue of EP groups was positively correlated with the mRNA of TLR4 and NF-κB.Conclusion The excessive activation of TLR4/NF-κB pathway in EP is associated with abnormal secretion of inflammatory cytokine IL-6.It is suggested that the local active inflammatory response and immune imbalance may be related to the occurrence and development of endometrial polyps.

关 键 词：子宫内膜息肉 TOLL样受体4 核因子ΚB 白细胞介素(IL)-6 

分 类 号：R711.74[医药卫生—妇产科学]