miR-120对台湾乳白蚁抵御绿僵菌的免疫反应的影响  

Impact of miR-120 on the immune response of Coptotermes formosanus against Metarhizium anisopliae

作　　者：张泓 李志强 贾凤龙[1] 陈伟文 ZHANG Hong;LI Zhi-Qiang;JIA Feng-Long;CHEN Wei-Wen(College of Life Science,Sun Yat-sen University,Guangzhou 510275,China;Institute of Zoology,Guangdong Academy of Sciences,Guangdong Public Laboratory of Wild Animal Conservation and Utilization,Guangdong Key Laboratory of Animal Conservation and Resource Utilization,Guangzhou 510260,China)

机构地区：[1]中山大学生命科学学院,广州510275 [2]广东省科学院动物研究所,广东省动物保护与资源利用重点实验室,广东省野生动物保护与利用公共实验室,广州510260

出　　处：《环境昆虫学报》2024年第4期948-957,共10页Journal of Environmental Entomology

基　　金：广州市科技重点项目(201904020002);广州市科技项目(2023A04J0147)。

摘　　要：本研究探究了miR-120在台湾乳白蚁Coptotermes formosanus抵御绿僵菌Metarhizium anisopliae的免疫反应中的作用。研究发现通过对台湾乳白蚁注射miR-120 agomir以增加白蚁体内miR-120的含量后,台湾乳白蚁的存活率降低约35%,绿僵菌对台湾乳白蚁的致死率提升约55%,同时显著抑制了台湾乳白蚁对绿僵菌的趋避反应。双荧光素酶报告系统等试验证实了miR-120靶向结合免疫基因Draper。通过对Draper进行注射法RNAi后,得到了类似于注射miR-120 agomir后的结果。研究结果表明,miR-120通过抑制靶基因Draper的表达,抑制了台湾乳白蚁抵御绿僵菌的免疫反应,最终导致台湾乳白蚁的存活率显著下降。该结果有助于进一步理解白蚁的免疫机制,并为开发抑制白蚁免疫防御反应的技术方法提供理论基础,有助于促进白蚁生物防治的发展。This study investigated the role of miR-120 in the immune response of Coptotermes formosanus against Metarhizium anisopliae through a series of experiments.First,injecting C.formosanus with miR-120 agomir to increase the level of miR-120 in C.formosanus decreased the survival rate of C.formosanus by about 35%and increased the mortality rate of C.formosanus treated with M.anisopliae to about 55%.At the same time,the repellent response of C.formosanus to M.anisopliae was significantly inhibited.Following,some experiments including the dual luciferase reporter system were performed to confirm that miR-120 targets the immune gene Draper.Finally,by injecting RNAi to knockdown of Draper and obtained results similar to those obtained after injection of miR-120 agomir.These results suggested that miR-120 inhibits the immune response of C.formosanus to M.anisopliae by inhibiting the expression of the target gene Draper,which ultimately leaded to a significant decrease in the survival rate of C.formosanus.This study will help us to better understand the immune mechanism of termites and provide a theoretical basis for the development of technical methods to inhibit termite immune responses,which will help to promote the development of biological control of termites.

关 键 词：台湾乳白蚁 miR-120 免疫反应 绿僵菌 白蚁防治 

分 类 号：Q968.1[生物学—昆虫学] S433[农业科学—农业昆虫与害虫防治]