多伞阿魏化学成分研究  

Studies on the chemical composition of Ferula feruloides

作　　者：毕英赫 庞克坚 李卉子 叶尔兰·巴合特江 木古丽·木哈西 胡燕[1] 杨新洲[1] BI Ying-he;PANG Ke-jian;LI Hui-zi;BAHETJAN Yerlan;MUHAXI Muguli;HU Yan;YANG Xin-zhou(College of Pharmacy,South-Central Minzu University,Wuhan 430074,China;Yili Normal University,Yining 835000,China)

机构地区：[1]中南民族大学药学院,湖北武汉430074 [2]伊犁师范大学,新疆伊宁835000

出　　处：《药学学报》2024年第7期2069-2076,共8页Acta Pharmaceutica Sinica

基　　金：新疆维吾尔自治区区域协同创新专项上海合作组织科技伙伴计划及国际科技合作计划项目(2023E01019);国家自然科学基金资助项目(82374144,82160796)。

摘　　要：综合应用正相硅胶柱层析、Sephadex LH-20葡聚糖凝胶柱色谱以及半制备型液相色谱等技术,对多伞阿魏[Ferula feruloides(Steudel)Korovin]根80%乙醇提取物的乙酸乙酯部位进行分离纯化,得到11个化合物,再运用现代波谱学方法(NMR、MS、UV、IR)鉴定化合物的结构,分别鉴定为baigene D(1)、baigene E(2)、baigene F(3)、β-kirialovin(4)、α-kirialovin(5)、falcarindiol(6)、ammoresinol(7)、dshamirone(8)、2,3-dihydro-7-hydroxy-2S^(*),3R^(*)-dimethyl-3-[4-methyl-5-(4-methy1-2-furyl)-3(E)-pentenyl]-furo[3,2-c]coumarin(9)、2,3-dihydro-7-hydroxy-2S^(*),3R^(*)-dimethyl-2-[4,8-dimethyl-3(E),7-nonadi-enyl]-furo[3,2-c]coumarin(10)和baigene C(11)。其中化合物1~3为新的香豆素类化合物,化合物4~6为首次从多伞阿魏中分离得到。采用MTT试验对化合物5、7~11的抗人胃癌(MKN-45)细胞增殖活性进行评价,结果表明,化合物7~11均表现出较强的抑制活性,化合物5表现出较弱的抑制活性。Eleven compounds were isolated and purified from the ethyl acetate part of 80%ethanol extract of Ferula feruloides root by a combination of normal-phase silica gel column chromatography,Sephadex LH-20 dextran gel column chromatography and semi-preparative liquid chromatography and then modern wave spectrometry methods(NMR,MS,UV,IR)were used to identify the structures of the compounds,which were identified as baigene D(1),baigene E(2),baigene F(3),β-kirialovin(4),α-kirialovin(5),falcarindiol(6),ammoresinol(7),dshamirone(8),2,3-dihydro-7-hydroxy-2S^(*),3R^(*)-dimethyl-3-[4-methyl-5-(4-methy1-2-furyl)-3(E)-pentenyl]-furo[3,2-c]coumarin(9),2,3-dihydro-7-hydroxy-2S^(*),3R^(*)-dimethyl-2-[4,8-dimethyl-3(E),7-nonadi-enyl]-furo[3,2-c]coumarin(10),and baigene C(11).Compounds 1−3 are new coumarin analogues,and compounds 4−6 are firstly isolated from F.feruloides.The anti-proliferative activity of compounds 5,7−11 against human gastric cancer(MKN-45)cells was evaluated using MTT assay,which showed that compounds 7−11 exhibited strong inhibitory activity,and compound 5 exhibited weak inhibitory activity.

关 键 词：阿魏属 多伞阿魏 化学成分 香豆素 倍半萜 

分 类 号：R284.1[医药卫生—中药学]