采用DNA条形码技术鉴定口岸输入性鼠形动物  

Application of DNA barcoding technology for identification of imported murine animal at ports

作　　者：孙玉婷 田锋 易海华 杨国平 张荣 田玲玲 孟媛 杨庆贵 耿合员 SUN Yuting;TIAN Feng;YI Haihua;YANG Guoping;ZHANG Rong;TIAN Lingling;MENG Yuan;YANG Qinggui;GENG Heyuan(Xinjiang International Travel Healthcare Center(Urumqi Customs Port Outpatient Department),Urumqi,Xinjiang 830011,China;不详)

机构地区：[1]新疆国际旅行卫生保健中心(乌鲁木齐海关口岸门诊部),新疆乌鲁木齐830011 [2]江苏国际旅行卫生保健中心(南京海关口岸门诊部)

出　　处：《中国国境卫生检疫杂志》2024年第3期247-249,共3页Chinese Journal of Frontier Health and Quarantine

基　　金：国家重点研发计划项目(2021YFC2301200);新疆维吾尔自治区自然科学基金项目(2023D01A87);海关总署科研项目(2023HK022);南京海关科研项目(2023KJ02)。

摘　　要：目的针对形态上难以识别的境外输入性鼠形动物,探索DNA条形码技术鉴定鼠种的可行性。方法江苏口岸收集1份输入性且在形态上难以鉴别的鼠形动物,通过PCR法扩增线粒体细胞色素C氧化酶Ⅰ基因(COI),目的片段经测序后进行序列比对分析,计算物种种间和种内的遗传距离,采用邻近法构建系统发育树。结果序列比对显示该输入性鼠形动物为黑家鼠(Rattus rattus);系统发育分析显示,物种种内遗传距离为7.7%,种间距离为11.6%~21.0%,该输入性鼠形动物在进化树上形成独立的进化分支。结论DNA条形码技术能准确鉴定输入性鼠形动物,尤其是有助于提高在形态上难以鉴别的鼠形动物鉴定的准确性。Objective To explore the feasibility of DNA barcoding technique for identification of rat species,which is difficult to identify morphologically.Methods One murine animals imported from Jiangsu ports that was morphologically difficult to identify was collected.Mitochondrial cytochrome C oxidase I(COI)gene was amplified by PCR method,the target fragment was sequenced for sequence comparison analysis,and the genetic distance between species and within species was calculated.Results The sequence comparison showed that the imported rodent was Rattus rattus.Phylogenetic analysis showed that the intraspecific genetic distance was 7.7%,and the interspecific genetic distance was 11.6%~21.0%.The imported murine animal formed one independent evolutionary branch on the evolutionary tree.Conclusion DNA barcoding technology could accurately and effectively identify imported murine animals,especially help to improve the accuracy of identification of morphologically difficult murine animals.

关 键 词：DNA条形码 输入性 鼠形动物 鉴定 

分 类 号：R183.5[医药卫生—流行病学]