丙泊酚和七氟醚对大鼠创伤性脑损伤后氧化应激的影响  

The effects of propofol and sevoflurane on neurological function after traumatic brain injury in rats

作　　者：杨立斌[1] 吴倩 黄莉 王婷[1] 高喜文[1] 刘辉[1] 孙龙 YANG Libin;WU Qian;HUANG Li;WANG Ting;GAO Xiwen;LIU Hui;SUN Long(Department of Anesthesia and Pain,the First People′s Hospital of Shizuishan Affiliated to Ningxia Medical University,Shizuishan 750021,China;Department of Stomatology,Ningxia Workers Rehabilitation Hospital,Yinchuan 750021,China)

机构地区：[1]宁夏医科大学附属石嘴山第一人民医院手术麻醉疼痛科,宁夏石嘴山753200 [2]宁夏工人疗养院口腔科,宁夏银川750021

出　　处：《宁夏医学杂志》2024年第7期561-564,共4页Ningxia Medical Journal

基　　金：宁夏科技支撑计划项目(2016KJHM114);宁夏医科大学校级项目(XM2018189)。

摘　　要：目的观察丙泊酚和七氟醚对大鼠创伤性脑损伤(TBI)后氧化应激的影响。方法将24只SD大鼠随机分为4组(n=6),即对照组、损伤组、损伤+50 mg/kg丙泊酚组(I+丙泊酚组)、损伤+2%七氟醚(呼气末浓度)组(I+七氟醚组),采用改良的Feeney法建立TBI模型。记录每组大鼠的平均动脉压和心率,检测血清中神经特异性血清生物标志物S-100β、神经元特异性烯醇化酶(NSE)和髓鞘碱性蛋白(MBP)的血液浓度。通过检测脑组织中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和丙二醛(MDA),明确脑组织中氧化应激水平。结果丙泊酚和七氟醚在麻醉维持期间对MAP、HR的影响差异无统计学意义(P>0.05);与对照组相比,损伤组大鼠血清中S-100β、MBP和NSE水平显著升高。丙泊酚和七氟醚在损伤后60 min显著降低了大鼠血清中S-100β、MBP和NSE的水平;丙泊酚和七氟醚都降低了TBI大鼠脑组织中MDA的表达,同时提高了SOD和CAT的水平,与对照组相比I+七氟醚组MDA、SOD、CAT差异有统计学意义(P<0.05)。结论丙泊酚和七氟醚对大鼠创伤性脑损伤后氧化应激具有保护作用,丙泊酚和七氟醚通过减少活性氧来改善TBI后大鼠的预后。Objective To observe the effects of propofol and sevoflurane on neurological function after traumatic brain injury(TBI)in rats.Methods SD rats were randomly divided into four groups(n=6):sham surgery group,injury group,injury+50 mg/kg propofol group(I+propofol),injury+2%sevoflurane group(I+sevoflurane).The TBI model was established using the improved Feeney method.Recorded the average arterial pressure and heart rate of each group of rats and measure the blood concentrations of neural specific serum biomarkers S-100β,neuron specific enolase(NSE),and myelin basic protein(MBP)in the serum.The level of oxidative stress was tested in brain tissue by detecting superoxide dismutase(SOD),catalase(CAT),and malondialdehyde(MDA)in brain tissue.Results There was no statistically significant difference in the effects of propofol and sevoflurane on MAP and HR during anesthesia maintenance(P>0.05);Compared with the control group,the levels of S-100β,MBP and NSE in the serum of rats in the injury group were significantly increased.Propofol and sevoflurane significantly reduced the levels of S-100β,MBP,and NSE in rat serum 60 minutes after injury;Both propofol and sevoflurane reduced the expression of MDA in the brain tissue of TBI rats,while increasing the levels of SOD and CAT.Compared with the control group,the difference in MDA,SOD,and CAT in the I+sevoflurane group was statistically significant(P<0.05).Conclusion Propofol and sevoflurane have a protective effect on neurological function after traumatic brain injury in rats.Propofol and sevoflurane improve the prognosis of TBI rats by reducing reactive oxygen species.

关 键 词：创伤性脑损伤 丙泊酚 七氟醚 

分 类 号：R651.15[医药卫生—外科学]