海南黄瓜抗枯萎病嫁接砧木筛选研究  

Study on Screening of Fusarium Wilt Resistant Grafting Rootstock of Cucumis sativus L. from Hainan Province

作　　者：王铃钰 邢梦玉[1,2] 张旭阳 易秋霞 伍晨溪 陈艳丽 朱国鹏[1,2] WANG Lingyu;XING Mengyu;ZHANG Xuyang;YI Qiuxia;WU Chenxi;CHEN Yanli;ZHU Guopeng(Nanfan College of Hainan University(Sanya Nanfan Research Institute of Hainan University),Sanya Hainan 572025,China;Key Laboratory for Quality Control of Tropical Horticultural Crops in Hainan Province,College of Tropical Agriculture and Forestry,Hainan University,Haikou 570228,China)

机构地区：[1]海南大学南繁学院(海南大学三亚南繁研究院),海南三亚572025 [2]海南省热带园艺作物品质调控重点实验室,海南大学热带农林学院,海口570228

出　　处：《种子》2024年第7期59-64,共6页Seed

基　　金：海南省重大科技计划项目(ZDKJ2021005)。

摘　　要：为筛选适宜海南冬春季节黄瓜抗枯萎病的嫁接砧木品种,对收集的21种砧木进行黄瓜枯萎病抗性鉴定,以易感砧木种质‘全福太郎’为对照,采用人工接种尖孢镰刀菌黄瓜专化型,鉴定各砧木的黄瓜枯萎病抗性。通过测定不同砧木的发病率、病情指数、根系活力、叶绿素相对含量、相对电导率、丙二醛含量等生理指标,结合新引进的病害严重程度指数曲线下面积(AUDRC)综合评价不同砧木对黄瓜枯萎病的抗性。结果表明,不同砧木对黄瓜枯萎病的抗性表现不同,病情指数评价指标表明,‘双力铁甲’‘金甲砧’‘创凡一号’为抗性砧木,‘金甲砧’AUDRC指标表现最好;生理指标中,根系活力表现较好为‘双力铁甲’‘金甲砧’‘创凡一号’;叶绿素含量较高的品种为‘金甲砧’‘创凡一号’‘日系冬之助’,显著高于对照组;相对电导率较低,为‘青根砧王’‘创凡一号’;丙二醛含量较低的为‘双力铁甲’‘金甲砧’‘创凡301’,显著低于其他砧木。依据病情指数、根系活力、SPAD、REC、MDA等生理指标,‘双力铁甲’‘金甲砧’‘创凡一号’为抗性砧木。研究表明,‘金甲砧’为21个砧木品种中综合抗黄瓜枯萎病最好的砧木。In order to screen out the varieties of grafted rootstocks suitable for cucumber(Cucumis sativus L.) wilt resistance in Hainan winter and spring, the resistance of cucumber wilt resistance of 21 kinds of rootstocks collected was identified. Using the susceptible rootstock germplasm 'Quanfutailang' as the control, the cucumber with Fusarium oxysporum was inoculated artificially to identify the cucumber wilt resistance of each rootstock. The resistance of different rootstocks to cucumber wilt was comprehensively evaluated by measuring the incidence, disease index, root activity, relative chlorophyll content, relative electrical conductivity, malondialdehyde content and other physiological indexes, combined with the newly introduced area under the index curve of disease severity(AUDRC). The results showed that the resistance of different rootstocks to cucumber wilt was different. The evaluation indexes of disease index showed that 'Shuangli Tiejia', 'Jinjiazhen' and 'Chuangfan No.1' were resistant rootstocks, and 'Jinjiazhen' had the best AUDRC index. Among the physiological indexes, the best performance of root activity was 'Shuanglitiejia', 'Jinjiazhen' and 'Chuangfan No.1', The varieties with higher chlorophyll content were 'Jinjiazhen', 'Chuangfan No.1' and 'Japanese Dongzhizhu', which were significantly higher than the control group. Low relative conductivity for 'Qinggenzhen King' 'Chuangfan No.1', The lower content of malondialdehyde was 'Shuanglitiejia', 'Jinjiazhen' and 'Chuangfan 301', which was significantly lower than other rootstocks. According to the physiological indexes such as disease index, root activity, SPAD, REC, MDA, etc., 'Shuanglitiejia', 'Jinjiazhen' and 'Chuangfan No.1' were resistant rootstocks. Further combined with AUDRC, the research showed that 'Jinjiazhen' was the best rootstock for comprehensive resistance to cucumber wilt among 21 rootstocks.

关 键 词：黄瓜枯萎病 砧木 抗性鉴定 病情指数 AUDRC 

分 类 号：S642.2[农业科学—蔬菜学]