外源褪黑素对碱胁迫下谷子萌发期生长和生理的影响  

Effects of Exogenous Melatonin on Growth and Physiology of Setaria italica var. germanica During Germination under Alkali Stress

作　　者：刘畅 徐明丽 吴柏辰 郭彦颉 尹佳乐 张文博 殷佳琪 苗兴芬 LIU Chang;XU Mingli;WU Baichen;GUO Yanjie;YIN Jiale;ZHANG Wenbo;YIN Jiaqi;MIAO Xingfen(Heilongjiang Bayi Agricultural University Agricultural College/Heilongjiang Province Key Laboratory of Modern Agricultural Cultivation Technology and Crop Quality Improvement,Daqing Heilongjiang 163319,China;Key Laboratory of Green and Low-carbon Agriculture in Northeast Plain,Ministry of Agriculture and Rural Aflairs,Daqing Heilongjiang 163319,China)

机构地区：[1]黑龙江八一农垦大学农学院/黑龙江省现代农业栽培技术与作物种质改良重点实验室,黑龙江大庆163319 [2]农业农村部东北平原农业绿色低碳重点实验室,黑龙江大庆163319

出　　处：《种子》2024年第7期65-71,80,共8页Seed

基　　金：黑龙江省博士后启动项目(LBH-Q17140);黑龙江省重点研发项目(GA21B009)。

摘　　要：为探究外源褪黑素对谷子碱胁迫的缓解效应,以5种耐碱性不同的谷子为材料,研究不同浓度(50、100、200、300、400μmol/L)褪黑素对25 mmol/L的NaHCO_(3)、Na_(2)CO_(3)(摩尔比1∶1)混合胁迫下谷子萌发生长和生理特性的影响。结果表明,碱胁迫显著抑制谷子萌发与幼苗生长,施加不同浓度外源褪黑素均能缓解碱胁迫对谷子生长的抑制作用,其中50μmol/L褪黑素对碱极敏感品种金谷1号缓解效果较为明显,而抗氧化酶活性则是中等品种长农35表现较明显,表明在碱胁迫下,褪黑素通过增加抗氧化酶活性,清除细胞内过量活性氧,维持细胞稳定性,从而提高谷子耐碱性。LIU Chang;XU Mingli;WU Baichen;GUO Yanjie;YIN Jiale;ZHANG Wenbo;YIN Jiaqi;MIAO Xingfen;Heilongjiang Bayi Agricultural University Agricultural College/Heilongjiang Province Key Laboratory of Modern Agricultural Cultivation Technology and Crop Quality Improvement;Key Laboratory of Green and Low-carbon Agriculture in Northeast Plain,Ministry of Agriculture and Rural Aflairs;In order to explore the alleviating effect of exogenous melatonin on alkali stress of Setaria italica var. germanica, five kinds of Setaria italica var. germanica with different alkaline tolerance were used as materials to study the effects of different concentrations of melatonin(50, 100, 200, 300, 400 μmol/L) on the germination, growth and physiological characteristics of Setaria italica var. germanica under 25 mmol/L NaHCO_(3) and Na_(2)CO_(3)(mixed molar ratio of 1 to 1) stress. The results showed that alkali stress significantly inhibited the germination and seedling growth of Setaria italica var. germanica, and different concentrations of exogenous melatonin could alleviate the inhibitory effect of alkali stress on Setaria italica var. germanica growth, among which 50 μmol/L melatonin was more effective on alkali-sensitive variety Jindu1, while the antioxidant enzyme activity of medium variety Changnong35 was more obvious, indicating that under alkali stress, melatonin could increase the activity of antioxidant enzymes, remove excess reactive oxygen species in cells, maintain cell stability, and improve the alkali tolerance of Setaria italica var. germanica.

关 键 词：谷子 碱胁迫 褪黑素 萌发期 

分 类 号：S515[农业科学—作物学]