雀啄灸对湿热痹阻型类风湿关节炎大鼠证候特征及滑膜细胞超微结构的影响  

Effects of pecking moxibustion on pattern characteristics and synovial cell ultrastructure of rats with rheumatoid arthritis due to damp heat affecting bones/joints

作　　者：岳婷 杨东宇 邓慧榕 刘煜 王煜 杨吉勃 赵中亭 严兴科 朱田田 YUE Ting;YANG Dongyu;DENG Huirong;LIU Yu;WANG Yu;YANG Jibo;ZHAO Zhongting;YAN Xingke;ZHU Tiantian(Gansu University of Chinese Medicine,Lanzhou 730000,China;Gansu Provincial Hospital of TCM,Lanzhou 730000,China)

机构地区：[1]Gansu University of Chinese Medicine,Lanzhou 730000,China [2]Gansu Provincial Hospital of TCM,Lanzhou 730000,China

出　　处：《Journal of Acupuncture and Tuina Science》2024年第4期280-288,共9页针灸推拿医学（英文版）

基　　金：国家自然科学基金委员会地区基金项目,No.82060892,No.81560791;甘肃省科技厅省级科技计划项目,No.20JR10RA313.

摘　　要：目的:观察雀啄灸对湿热痹阻型类风湿关节炎(RA)大鼠病变关节红肿热痛证候特征和滑膜细胞超微结构的影响,探讨雀啄灸干预早期RA的抗炎机制。方法:从78只雌性大鼠中随机抽取18只为空白组,其余大鼠采用“胶原诱导性关节炎加风、湿、热环境刺激”的复合造模法复制“病证结合”湿热痹阻型RA大鼠模型。将造模成功的54只大鼠随机分为模型组、药物组和雀啄灸组,每组18只。药物组大鼠于第1、8和15天按照1 mg/(kg·bw)剂量给予甲氨蝶呤灌胃治疗。雀啄灸组大鼠曲池、大椎和阿是穴进行雀啄灸治疗,每穴每日施灸15 min,6 d为一疗程,共3个疗程。治疗结束后观察各组大鼠病变关节局部毛细血管通透性、关节肿胀度、关节表面温度及足底热痛阈值,并采用透射电镜技术观察各组大鼠滑膜细胞超微结构改变。结果:与空白组相比,模型组大鼠病变关节局部肿胀度、表面温度和伊文思蓝(EB)漏出量均明显升高(P<0.05),热痛阈值明显降低(P<0.05),滑膜细胞超微结构出现明显病理损伤。与模型组相比,药物组和雀啄灸组大鼠病变关节肿胀度、表面温度和EB漏出量均明显降低(P<0.05),热痛阈值明显升高(P<0.05),滑膜细胞超微结构异常得到明显改善。与药物组相比,雀啄灸组大鼠热痛阈值改善更为明显(P<0.05)。结论:雀啄灸可明显改善湿热痹阻型RA大鼠病变关节局部红、肿、热、痛的证候特征,且能有效修复损伤的滑膜超微结构;提示雀啄灸干预对早期RA具有显著的抗炎作用。Objective:To observe the effect of pecking moxibustion on the pattern characteristics of redness,swelling,heat,and pain in the affected joints,also the synovial cell ultrastructure in rats with rheumatoid arthritis(RA)due to damp heat affecting bones/joints,and to explore the anti-inflammatory mechanism of pecking moxibustion in treating the early stage of RA.Methods:Eighteen rats were randomly selected from 78 female ones as the blank group,and all the other rats were subjected to preparing the“differentiation of disease and pattern”RA model due to damp heat affecting bones/joints by using the method of“collagen-induced arthritis plus windy,damp,and hot environment stimulation”.Fifty-four rats with successful modeling were randomly divided into a model group,a drug group,and a pecking moxibustion group,with 18 rats in each group.Rats in the drug group were given methotrexate at a dose of 1 mg/(kg·bw)on the 1st,8th,and 15th days.Rats in the pecking moxibustion group were treated with pecking moxibustion at Quchi(LI11),Dazhui(GV14),and Ashi points,and each point was treated with moxibustion for 15 min every day and a total of 3 courses of treatment,with 6 d as a course of treatment.After treatment,the capillary permeability,joint swelling,joint surface temperature,and plantar thermal pain threshold of the diseased joints in rats were observed,and the ultrastructural changes of synovial cells were observed by transmission electron microscopy.Results:The local swelling,surface temperature,and Evans blue(EB)leakage volume were significantly higher(P<0.05),the thermal pain threshold was significantly lower(P<0.05),and the synovial cell ultrastructure was obviously damaged in the affected joints in the model group compared with the blank group.The swelling degree,surface temperature,and EB leakage volume were significantly reduced(P<0.05),the thermal pain threshold was significantly increased(P<0.05),and the ultrastructural abnormalities of synovial cells were significantly improved in the diseased joints in t

关 键 词：灸法 雀啄灸 关节炎 类风湿 湿热痹阻证 滑膜细胞 大鼠 

分 类 号：R2-03[医药卫生—中医学]