Cell membrane vesicles derived from hBMSCs and hUVECs enhance bone regeneration  

作　　者：Dandan Wang Yaru Guo Boon Chin Heng Xuehui Zhang Yan Wei Ying He Mingming Xu Bin Xia Xuliang Deng 

机构地区：[1]Department of Pediatric Dentistry,Peking University School and Hospital of Stomatology,Beijing 100081,P.R.China [2]Department of Geriatric Dentistry,Peking University School and Hospital of Stomatology,National Center for Stomatology,National Clinical Research Center for Oral Diseases,National Engineering Research Center of Oral Biomaterials and Digital Medical Devices,Beijing 100081,P.R.China [3]Department of Dental Materials&Dental Medical Devices Testing Center,Peking University School and Hospital of Stomatology,National Engineering Research Center of Oral Biomaterials and Digital Medical Devices,Beijing 100081,P.R.China

出　　处：《Bone Research》2024年第2期361-371,共11页骨研究（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.U22A20160,82221003,81991505,82201123);China Postdoctoral Science Foundation(2022M720290,2021M700279);Hubei Provincial Key Laboratory of Oral and Maxillofacial Development and Regeneration Open Fund(2022kqhm004).

摘　　要：Bone tissue renewal can be enhanced through co-transplantation of bone mesenchymal stem cells(BMSCs)and vascular endothelial cells(ECs).However,there are apparent limitations in stem cell-based therapy which hinder its clinic translation.Hence,we investigated the potential of alternative stem cell substitutes for facilitating bone regeneration.In this study,we successfully prepared cell membrane vesicles(CMVs)from BMSCs and ECs.The results showed that BMSC-derived cell membrane vesicles(BMSC-CMVs)possessed membrane receptors involved in juxtacrine signaling and growth factors derived from their parental cells.EC-derived cell membrane vesicles(EC-CMVs)also contained BMP2 and VEGF derived from their parental cells.BMSC-CMVs enhanced tube formation and migration ability of hUVECs,while EC-CMVs promoted the osteogenic differentiation of hBMSCs in vitro.Using a rat skull defect model,we found that co-transplantation of BMSC-CMVs and EC-CMVs could stimulate angiogenesis and bone formation in vivo.Therefore,our research might provide an innovative and feasible approach for cell-free therapy in bone tissue regeneration.

关 键 词：hinder APPARENT vesicles 

分 类 号：R336[医药卫生—人体生理学]