基于RNA测序技术鉴定和分析绒山羊毛囊不同生长期mRNA的可变剪接  

Identification and Analysis of Alternative Splicing of mRNA Related to Different Growth Stages of Cashmere Goat Hair Follicles Based on RNA Sequencing Technology

作　　者：李莹莹 董华娇 梁千千 张洁[1] 李甜甜 许赫 马月辉[2] 韩吉龙 杨敏 LI Yingying;DONG Huajiao;LIANG Qianqian;ZHANG Jie;LI Tiantian;XU He;MAYuehui;HAN Jilong;YANG Min(College of Animal Science and Technology,Shihezi University,Shihezi 832003,China;Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193,China)

机构地区：[1]石河子大学动物科技学院,石河子832003 [2]中国农业科学院北京畜牧兽医研究所,北京100193

出　　处：《中国草食动物科学》2024年第4期1-7,共7页China Herbivore Science

基　　金：国家自然基金项目(32060745,31802045);石河子大学国际科技合作推进计划项目(GJHZ202307)。

摘　　要：可变剪接(Alternative splicing,AS)是在转录后水平调控基因表达的一种重要方式。为挖掘调控绒山羊毛囊周期性生长相关的关键可变剪接,通过比较绒山羊绒毛不同生长时期的皮肤转录组数据,以P<0.05,|Inc‐LevelDifference|>0.1为标准筛选差异的可变剪接,并进行功能分析。结果显示,转换期Ⅰ、Ⅱ、Ⅲ、Ⅳ中分别鉴定到1615、1667、1387和1522个差异可变剪接,分别对应了1202、1268、1073、1170个基因;这些基因主要富集于Notch、Hedgehog、mTOR、AMPK、PI3K-Akt、Wnt、TGF-β等与毛囊发育相关的信号通路;蛋白互作网络结合富集分析表明,Ctnnb1、Gsk3b、Tgfbr1、Vegfa、Gli1等基因产生的可变剪接可能参与调控毛囊周期性生长。综上可知,可变剪接在绒山羊毛囊周期性生长过程中普遍存在,且在不同发育阶段间存在差异;鉴定到调控绒山羊毛囊周期性生长发育相关的关键差异可变剪接基因及其重要信号通路。Alternative splicing(AS)plays a crucial role in regulating gene expression post-transcriptionally.To explore key alternative splicing events linked to the cyclical growth of cashmere goat hair follicles,the skin transcriptome data of different growth stages were compared and analyzed.The results showed that 1615,1667,1387 and 1522 differential alternative splicing were identified in transition periodsⅠ,Ⅱ,ⅢandⅣ,respectively,corresponding to 1202,1268,1073 and 1170 genes.These genes were predominantly enriched in signaling pathways such as Notch,Hedgehog,mTOR,AMPK,PI3K-Akt,Wnt,TGF-β,all known to be related to hair follicle development.Protein interaction network analysis coupled with enrichment analysis showed that alternative splicing produced by genes such as Ctnnb1,Gsk3b,Tgfbr1,Vegfa,Gli1 may contribute to the regulation of hair follicle cycle growth.In summary,alternative splicing is prevalent during the cyclical growth of cashmere goat hair follicles,with notable differences across developmental stages.The key differentially alternative splicing genes and their related signaling pathways were identified,which provided valuable insights into the cycle growth and development of hair follicles in cashmere goats.

关 键 词：绒山羊 毛囊周期性生长 可变剪接 转录组测序 

分 类 号：S827.2[农业科学—畜牧学]