截短侧耳素衍生物PCI对48株牛源耐甲氧西林金黄色葡萄球菌临床菌株的抗菌活性  

Antibacterial Activity of Pleuromutilin Derivative PCI Against 48 Clinical Isolates of Bovine Methicillin-resistant Staphylococcus Aureus

作　　者：马丹倩 张贺超 齐显龙 李剑勇[1] 李准 MA Danqian;ZHANG Hechao;QI Xianlong;LI Jianyong;LI Zhun(Key Lab of New Animal Drug of Gansu Province,Key Lab of Veterinary Pharmaceutical Development of Ministry of Agriculture and Rural Affairs,Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS,Lanzhou 730050,China)

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所,农业农村部兽用药物创制重点实验室,甘肃省新兽药重点实验室,兰州730050

出　　处：《中国草食动物科学》2024年第4期77-81,87,共6页China Herbivore Science

基　　金：中国农业科学院科技创新工程(25-LZIHPS-02)。

摘　　要：为了探讨截短侧耳素衍生物PCI对48株牛乳腺炎耐甲氧西林金黄色葡萄球菌临床菌株的体内外抗菌活性,采用微量肉汤稀释法测定了PCI对金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌(MRSA)标准菌株,以及48株牛乳腺炎耐甲氧西林金黄色葡萄球菌临床菌株的最小抑菌浓度(MIC),并进行了小鼠大腿感染试验。结果表明,PCI对金黄色葡萄球菌和MRSA的MIC均为0.0078μg/mL,对牛源MRSA临床菌株的MIC为0.0078~0.125μg/mL;小鼠大腿感染试验结果表明,PCI减轻了肌肉组织的变性和坏死,且大腿组织载菌量显著低于延胡索酸泰妙菌素组(P<0.05),极显著低于模型组(P<0.0001)。综上所述,PCI对48株牛乳腺炎耐甲氧西林金黄色葡萄球菌临床分离菌株具有优异的抗菌活性,能够减轻MRSA造成的大腿肌肉感染。In order to investigate in vitro and in vivo antibacterial activity of the truncated pleuromutilin derivative PCI against 48 clinical strains of methicillin-resistant Staphylococcus aureus in bovine mastitis,the minimal inhibitory concentration(MIC)of PCI against Staphylococcus aureus,methicillin-resistant Staphylococcus aureus(MRSA)standard strains,and 48 clinical strains of methicillin-resistant Staphylococcus aureus in bovine mastitis were determined by micro-broth dilution method,and the thigh infection experiment in mice was carried out.The results showed that the MIC of PCI against S.aureus and MRSA was 0.0078μg/mL,and the MIC of PCI against bovine MRSA clinical strains was 0.0078-0.125μg/mL.The results of thigh infection in mice showed that PCI reduced the degeneration and necrosis of muscle tissue,and the amount of bacteria in thigh tissue was significantly lower than that in the tiamulin fumarate group(P<0.05),which was significantly lower than that in the model group(P<0.0001).In summary,PCI has excellent antibacterial activity against 48 clinical isolates of methicillin-resistant Staphylococcus aureus in bovine mastitis,which can alleviate thigh muscle infections caused by MRSA.

关 键 词：截短侧耳素衍生物PCI 牛乳腺炎 耐甲氧西林金黄色葡萄球菌 

分 类 号：S853.7[农业科学—临床兽医学]