不同免疫检验法对乙肝病毒感染血清标志物检验效果的影响  

Effect of Different Immunoassays on the Detection of Serum Markers of Hepatitis B Virus Infection

作　　者：杨伦辉 戴红妹 YANG Lun-hui;DAI Hong-mei(Laboratory Department of Anyi County Center for Disease Control and Prevention,Anyi 330500,Jiangxi,China;Laboratory Department of Anyi County People's Hospital,Anyi 330500,Jiangxi,China)

机构地区：[1]安义县疾病预防控制中心检验科,江西安义330500 [2]安义县人民医院检验科,江西安义330500

出　　处：《医学信息》2024年第15期142-145,共4页Journal of Medical Information

摘　　要：目的研究不同免疫检验法对乙肝病毒感染血清标志物检验效果的影响。方法以2020年6月-2023年6月安义县人民医院收治的60例疑似乙肝患者为研究对象,分别采用化学发光免疫分析法(CLIA)与酶联免疫吸附试验(ELISA)检测患者的血清标志物[乙肝表面抗原(HBsAg)、乙肝表面抗体(HBsAb)、乙肝e抗原(HBeAg)、乙肝e抗体(HBeAb)、乙肝核心抗体(HBcAb)],比较两种检验方式对乙肝的诊断效能及对乙肝血清标志物的阳性检出率,统计其对不同乙肝分型的诊断准确率。结果CLIA对乙肝的诊断敏感度、准确度、特异度高于ELISA(P<0.05);CLIA对HBsAg、HBsAb、HBeAg的阳性检出率高于ELISA(P<0.05),但二者对HBcAb、HBeAb的阳性检出率比较,差异无统计学意义(P>0.05)。CLIA对乙肝小二阳和乙肝恢三阳的诊断准确率高于ELISA(P<0.05),但二者对乙肝大三阳、小三阳的诊断准确率比较,差异无统计学意义(P>0.05)。结论CLIA与ELISA在乙肝诊断中具有确切应用价值,其中CLIA诊断效能较高,其对血清标志物的检出效果更为理想。Objective To study the effect of different immune test methods on the test effect of serum markers of hepatitis B virus infection.Methods A total of 60 patients with suspected hepatitis B admitted to Anyi County People's Hospital from June 2020 to June 2023 were selected as the research objects.The serum markers[hepatitis B surface antigen(HBsAg),hepatitis B surface antibody(HBsAb),hepatitis B e antigen(HBeAg),hepatitis B e antibody(HBeAb),and hepatitis B core antibody(HBcAb)]were detected by chemiluminescence immunoassay(CLIA)and enzyme-linked immunosorbent assay(ELISA),respectively.The diagnostic efficacy of the two methods for hepatitis B and the positive detection rate of hepatitis B serum markers were compared.The diagnostic accuracy of different hepatitis B types was statistically analyzed.Results The sensitivity,accuracy and specificity of CLIA in the diagnosis of hepatitis B were higher than those of ELISA(P<0.05).The positive detection rates of HBsAg,HBsAb and HBeAg by CLIA were higher than those by ELISA(P<0.05),but there was no significant difference in the positive detection rates of HBcAb and HBeAb between CLIA and ELISA(P>0.05).The diagnostic accuracy of CLIA was higher than that of ELISA(P<0.05),but there was no significant difference in the diagnostic accuracy between between CLIA and ELISA(P>0.05).Conclusion CLIA and ELISA have definite application value in the diagnosis of hepatitis B.The diagnostic efficiency of CLIA is higher,and its detection effect on serum markers is more ideal.

关 键 词：乙肝 血清标志物 化学发光免疫分析法 酶联免疫吸附试验 乙肝表面抗原 

分 类 号：R446[医药卫生—诊断学]