灵菌红素对创面分离耐甲氧西林金黄色葡萄球菌的抑菌效果及作用机制  

Antibacterial activity of prodigiosin against methicillin-resistant Staphylococcus aureus isolated from wounds and its mechanism

作　　者：王蔚 邵平扬 郁勤龙 侯柏龙 沈伟锋 薛军 WANG Wei;SHAO Pingyang;YU Qinong;HOU Baiong;SHEN Weifeng;XUE Jun(Clinical Laboratory,The First Hospital of Jiaxing,Jiaxing 314000,China;不详)

机构地区：[1]嘉兴市第一医院检验科,314000 [2]嘉兴市第一医院骨科,314000

出　　处：《浙江医学》2024年第14期1531-1534,1539,共5页Zhejiang Medical Journal

基　　金：嘉兴市科技计划项目(2022AY30018)。

摘　　要：目的探讨灵菌红素对创面分离耐甲氧西林金黄色葡萄球菌(MRSA)的抑菌效果和作用机制。方法选取2021年至2022年嘉兴市第一医院分离培养的64株创面组织感染MRSA菌株,采用微量肉汤稀释法测定单用灵菌红素及与左氧氟沙星联合作用时的最低抑菌浓度(MIC),计算部分抑菌指数评价联合抑菌效果;并通过透射电镜观察灵菌红素对MRSA菌株的抑菌作用。结果灵菌红素对MRSA菌株的50%MIC为0.5 mg/L,90%MIC为1.0 mg/L;与左氧氟沙星联用时1株(1.54%)结果为协同作用,30株(46.15%)结果为相加作用;透射电镜观察到灵菌红素对MRSA菌株细胞膜结构完整性破坏作用明显。结论灵菌红素通过破坏细菌胞膜达到对MRSA菌株的抑菌作用,与左氧氟沙星联合使用时具有一定的协同或相加作用。Objective To investigate the antibacterial effect and its mechanism of prodigiosin on methicillin-resistant Staphylococcus aureus(MRSA)isolated from wounds.Methods A total of 64 strains of MRSA isolated from wound tissue infections of the First Hospital of Jiaxing from 2021 to 2022 were selected.The minimum inhibitory concentrations(MIC)of prodigiosin used alone and combined with levofloxacin were both determined by the micro-broth dilution method,and the fractional inhibitory concentration was calculated to evaluate the bacteriostatic effect of the combined use.The antibacterial mechanism of prodigiosin was observed by transmission electron microscopy.Results The 50%MIC and 90%MIC of prodigiosin against MRSA were 0.5 and 1.0 mg/L,respectively.The synergistic effect of prodigiosin and levofloxacin was observed in one strain(1.54%),and the additive effect was in 30 strains,accounting for 46.15%.It was observed by transmis-sion electron microscopy that prodigiosin had a significant effect on destroying the integrity of the cell membrane structure of MRSA strains.Conclusion Prodigiosin can inhibit MRSA by destroying the bacterial cell membrane and has a certain synergistic or additive effect when combined with levofloxacin.

关 键 词：灵菌红素 耐甲氧西林金黄色葡萄球菌 联合药敏 

分 类 号：R446.5[医药卫生—诊断学]