定量PCR法在病毒滴度检测中的应用进展  

Research progress of quantitative PCR for virus titer detection

作　　者：闫奥博(综述) 朱为(审校) Yan Aobo;Zhu Wei(No.3 Research laboratory,Shanghai Institute of Biological Products Co.,Ltd.,Shanghai 200051,China)

机构地区：[1]上海生物制品研究所有限责任公司第三研究室,上海200051

出　　处：《国际生物制品学杂志》2024年第3期169-174,共6页International Journal of Biologicals

摘　　要：定量PCR(quantitative PCR, qPCR)被广泛应用于mRNA表达、病原体检测和病毒滴定等领域, 具有快速、敏感、污染概率低和可重复的特点。此文主要对qPCR法检测各种病毒滴度的研究进展进行综述, 介绍qPCR在RNA病毒以及DNA病毒检测中的应用, 并与其他检测方法进行对比, 展现qPCR检测病毒滴度的应用前景。Quantitative PCR(qPCR)is widely used in various fields such as mRNA expression,pathogen detection,and viral titration,and is characterized by rapidity,high sensitivity,low contamination rate and reproducibility.This paper mainly reviews the research progress of qPCR in titer detection of various viruses,introduces the appplication of qPCR in detection of some RNA viruses and DNA virus,and compares it with other detection methods,to show the application prospect of qPCR for virus titer detection.

关 键 词：实时定量聚合酶链反应 DNA病毒 RNA病毒 滴度 

分 类 号：R446[医药卫生—诊断学]