基于谱效关系的续断盐制前后对肾阳虚大鼠的物质基础研究  

Material basis of kidney Yang deficiency rats before and after salt processing of Dipsacus asper based on spectrum-effect relationship

作　　者：方可儿 张海瑞 竹炳 叶宇 陈磊[1,2] 杜伟锋[1,2,3] 葛卫红 FANG Ke-er;ZHANG Hai-rui;ZHU Bing;YE Yu;CHEN Lei;DU Wei-feng;GE Wei-hong(School of Pharmaceutical Sciences,Zhejiang Chinese Medical University,Hangzhou 311402,China;Research Center of Traditional Chinese Medicine Processing Technology,Zhejiang Chinese Medical University,Hangzhou 311401,China;Zhejiang Chinese Medical University Chinese Medicine Yinpian Co.,Ltd.,Hangzhou 311401,China)

机构地区：[1]浙江中医药大学药学院,浙江杭州311402 [2]浙江中医药大学中药炮制技术研究中心,浙江杭州311401 [3]浙江中医药大学中药饮片有限公司,浙江杭州311401

出　　处：《中国中药杂志》2024年第12期3220-3228,共9页China Journal of Chinese Materia Medica

基　　金：国家重点研发计划项目(2018YFC1707001);杭州市农业与社会发展科研重点项目(202204A06);宁波市“科技创新2025”重大专项(20201ZDYF020069,2020Z089)。

摘　　要：研究续断盐制前后指纹图谱与温补肾阳药效之间的谱效关系,寻找续断盐制前后抗肾阳虚的主要活性成分,为明确盐制对肾阳虚药效的影响提供依据。采用HPLC-DAD建立了续断盐制前后HPLC指纹图谱,得到15个共有峰,并指认了11个成分。检测大鼠血清中多种成分含量的含量变化,比较续断盐制前后的药效差异。药理实验结果表明续断盐制后能够增强肾指数,同一剂量下,生续断和盐续断组间有显著差异;与模型组相比,给药组的大鼠血清中促肾上腺皮质激素(ACTH)、环磷酸腺苷(cAMP)、皮质酮(CORT)、雌二醇(E_2)、生长激素(GH)、Na^(+)-K^(+)-ATP酶(Na^(+)-K^(+)-ATPase)、睾酮(T)、甲状腺素(T4)含量均有一定程度上升,环磷酸鸟苷(cGMP)、肿瘤坏死因子(TNF-α)含量均有一定程度下降,其中生续断高剂量组、盐续断中剂量组、盐续断高剂量组和阳性药组大鼠血清中以上指标均有显著性差异,整体结果表明盐续断抗肾阳虚药效强于生续断。采用灰色关联分析、熵值法和皮尔逊相关性分析法对续断药效进行评价,筛选出盐制续断抗肾阳虚药效的成分。根据关联度排序变化结果得,续断盐制前后排名上升的成分为马钱苷、绿原酸、续断苷A、川续断皂苷Ⅵ、咖啡酸、异绿原酸B,初步推测这些化合物可能是续断盐制前后治疗肾阳虚的潜在药效成分。确定了续断盐制前后变化的成分,证明了盐续断在治疗肾阳虚方面优于续断,建立了续断盐制前后与治疗肾阳虚的药效之间的谱效关系,为后续对盐制续断的药效成分及分子机制研究奠定了基础。This paper aims to study the spectrum-effect relationship between the fingerprints before and after salt processing of Dipsacus asper and the efficacy of warming and tonifying kidney Yang and find the main active components against kidney Yang deficiency before and after salt processing of D.asper,so as to provide the basis for clarifying the effect of salt processing on kidney Yang deficiency.The HPLC fingerprint before and after salt processing of D.asper was established by the HPLC-DAD.15 common peaks were obtained,and 11 components were identified.The content changes of various components in rat serum were detected,and the difference in efficacy before and after salt processing was compared.The results of pharmacological experiments showed that salt processing of D.asper could enhance the kidney index.At the same dose,there was a significant difference between the raw D.asper and D.asper after salt processing groups.Compared with the model group,the contents of ACTH,cAMP,CORT,E2,GH,Na^(+)-K^(+)-ATPase,T,and T4 in the serum of rats in the administration group increased to a certain extent,and the contents of cGMP and TNF-αdecreased to a certain extent.Among them,there were significant differences in the above indexes in the serum of rats in the highdose group of raw D.asper,middle-dose group of D.asper after salt processing,high-dose group of D.asper after salt processing,and the positive drug group.The overall results showed that D.asper after salt processing was more effective than raw D.asper in preventing kidney yang deficiency.The efficacy of D.asper was evaluated by grey correlation analysis,entropy method,and Pearson correlation analysis,and the components of D.asper after salt processing against kidney yang deficiency were screened out.According to the results of correlation degree ranking,the components with increased ranking before and after salt processing of D.asper were loganin,chlorogenic acid,dipsacoside A,asperosaponinⅥ,caffeic acid,and isochlorogenic acid B.It was preliminarily speculated t

关 键 词：续断 盐制 谱效关系 指纹图谱 肾阳虚 灰色关联度 

分 类 号：R285.5[医药卫生—中药学]