桂枝水煎液的化学成分研究  

Chemical constituents from Cinnamomi Ramulus decoction

作　　者：刘青[1] 刘珍伶[2] 易立涛[1] LIU Qing;LIU Zhen-ling;YI Li-tao(Department of Chemical and Pharmaceutical Engineering,College of Chemical Engineering,Huaqiao University,Xiamen 361021,China;State Key Laboratory of Applied Organic Chemistry,Lanzhou University,Lanzhou 730000,China)

机构地区：[1]华侨大学化工学院制药工程系,福建厦门361021 [2]兰州大学功能有机分子化学国家重点实验室,甘肃兰州730000

出　　处：《中国中药杂志》2024年第12期3258-3262,共5页China Journal of Chinese Materia Medica

基　　金：福建省自然科学基金项目(2022J01315)。

摘　　要：采用RP-18反相柱色谱、硅胶柱色谱、Sephadex LH-20柱色谱以及半制备型高效液相色谱等手段对桂枝Cinnamomi Ramulus水煎液乙酸乙酯萃取物进行化学成分研究,从中分离得到6个化合物,通过现代波谱学分析,分别鉴定为4,5,10,11-tetrahydroxybisabol-7-ene(1)、4,5,10,11-tetrahydroxybisabolin(2)、1-苯基-1,2,3-丙三醇(3)、(+)-lyoniresinol(4)、苯甲酸(5)和decumbic acid(6)。其中化合物1为1个新的没药烷型倍半萜,化合物2、3为首次从桂枝中分离得到。对没药烷型倍半萜化合物2进行抗炎活性和人胰腺癌细胞(PANC-1细胞)的细胞毒活性测试,结果显示化合物2在40μmol·L^(-1)浓度时对NO的抑制率为32.9%;在20μmol·L^(-1)时对PANC-1细胞的生长抑制率为14.5%,未显示出明显活性。Six compounds were isolated from the ethyl acetate extracts of Cinnamomi Ramulus decoction by RP-18,silica gel,Sephadex LH-20 column chromatography,together with prep-HPLC methods.Based on HR-ESI-MS,MS,1D and 2D NMR spectral analyses,the structures of the six compounds were identified as 4,5,10,11-tetrahydroxybisabol-7-ene(1),4,5,10,11-tetrahydroxybisabolin(2),1-phenyl-1,2,3-glycerol(3),(+)-lyoniresinol(4),benzoic acid(5),and decumbic acid(6).Compound 1 was a new bisabolene-type sesquiterpene,and compounds 2 and 3 were isolated from the Cinnamomi Ramulus for the first time.Moreover,the bisabolene-type sesquiterpene(2)was assayed for its anti-inflammatory activity and cytotoxicity to human pancreatic cancer cells(PANC-1 cells).Results showed that compound 2 exhibited an inhibitory rate of 32.9%on nitric oxide(NO)at a dose of 40μmol·L^(-1) and a proliferation inhibition rate of 14.5%against PANC-1 cells at a dose of 20μmol·L^(-1).It did not demonstrate significant activity.

关 键 词：桂枝水煎液 化学成分 没药烷型倍半萜 抗炎活性 PANC-1细胞毒活性 

分 类 号：R284.1[医药卫生—中药学]