梅SDR基因家族的鉴定及在花开放阶段的表达分析  

Identification of SDR Gene Family in Prunus mume and Its Expression Analysis During Flower Opening Stage

作　　者：李玉 贾浩田 耿晓云 任红茜 郝瑞杰[2] LI Yu;JIA Haotian;GENG Xiaoyun;REN Hongqian;HAO Ruijie(College of Urban and Rural Construction,Shanxi Agricultural University,Jinzhong,030801;College of Horticulture,Shanxi Agricultural University,Jinzhong,030801)

机构地区：[1]山西农业大学城乡建设学院,晋中030801 [2]山西农业大学园艺学院,晋中030801

出　　处：《基因组学与应用生物学》2024年第6期997-1009,共13页Genomics and Applied Biology

基　　金：国家自然科学基金(31870696)资助。

摘　　要：本文旨在探究梅(Prunus mume)短链脱氢酶/还原酶(short-chain dehydrogenase/reductase,SDR)基因家族的生物信息学特征以及在花开放阶段的表达模式。通过生物信息学方法,从梅全基因组中鉴定出159个PmSDR基因,并进行保守基序、染色体定位、共线性关系和顺式作用元件分析,结合梅不同开花时期、花器官的转录组数据以及荧光定量PCR技术探究了PmSDR基因家族的表达模式。结果表明,PmSDR基因家族可分为4种类型,同一亚家族成员在保守基序方面具有较高的相似性;PmSDR基因家族不均匀分布在8条染色体上,并且存在大量成簇分布;种间共线性分析发现,与杏(Prunus armeniaca)相比,梅与桃(Prunus persica)SDR基因家族的同源性更高;该家族基因在启动子区域存在大量与光响应、植物生长发育、激素调节以及逆境响应有关的顺式作用元件。表达模式分析发现,PmSDR基因家族在不同开花时期和花器官的表达水平有较大差异,选择在开花时期表达量明显上调或在花器官中具有高表达量的PmSDR114C1、PmSDR114C16、PmSDR108E3、PmSDR108E9、PmSDR108E16、PmSDR108E17和PmSDR108E19进行荧光定量PCR验证,结果表明PmSDR114C1、PmSDR114C16可能参与梅的花期调控,PmSDR108E3、PmSDR108E9、PmSDR108E16、PmSDR108E17和PmSDR108E19可能参与梅的花香物质合成。本研究为探析PmSDR基因的功能提供了理论依据与参考,也为探讨梅开花阶段的分子机制提供了研究依据。The objective of this study is to explore the bioinformatics characteristics of the short-chain dehydrogenase/reductase(SDR)gene family and the function of the flowering process.The conserved motifs,chromosome localization,collinearity,evolutionary relationship and cis-acting elements of PmSDR gene family were analyzed by bioinformatics methods,and the expression pattern of PmSDR gene family were explored by combining transcriptome data of different flowering stages and flower organs and fluorescence quantitative PCR.A total of 159 PmSDR gene family members were identified and classified into 4 types and the members of the same subfamily had high conserved motifs.It is not evenly distributed on 8 chromosomes and has a large number of clusters.The interspecific collinearity analysis showed that compared with Prunus armeniaca,Prunus mume and Prunus persica had higher homology of SDR gene family.There are a large number of cis-acting elements involved in plant growth and development,hormone regulation,light response and stress response in the promoter region.Analysis of gene expression patterns showed that:PmSDR gene family are widely expressed in different flowering stages and flower organs,and their expression levels vary greatly.Seven genes,PmSDR114C1,PmSDR114C16,PmSDR108E3,PmSDR108E9,PmSDR108E17 and PmSDR108E19,which were significantly up regulated in the flowering period or had specific high expression in the flower organs,were selected for fluorescence quantitative verification analysis showed that PmSDR114C1 and PmSDR114C16 may be involved in the regulation of flowering period in Prunus mume.PmSDR108E3,PmSDR108E9,PmSDR108E16,PmSDR108E17 and PmSDR108E19 may be involved in the floral synthesis in Prunus mume.This study provides theoretical basis and reference for the function analysis and also provides research basis for the molecular mechanism of PmSDR in the process of blossom.

关 键 词：梅 SDR 开花过程 表达模式 

分 类 号：S685.99[农业科学—观赏园艺]