H_(2)O_(2)氧化-荧光法定量检测黑木耳黑色素  

Quantitative determination of melanin in Auricularia auricula by H_(2)O_(2)oxidation-fluorescence

作　　者：薛娇 张彦龙[1] 雷虹[1] 曾伟民[1] 苏林贺 XUE Jiao;ZHANG Yanlong;LEI Hong;ZENG Weimin;SU Linhe(Engineering Research Center of Agricultural Microbiology Technology,Ministry of Education/Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region/Key Laboratory of Microbiology,College of Heilongjiang Province,School of Life Sciences,Heilongjiang University,Harbin 150080,China)

机构地区：[1]黑龙江大学生命科学学院农业微生物技术教育部工程研究中心/黑龙江省寒地生态修复与资源利用重点实验室/黑龙江省普通高校微生物重点实验室,哈尔滨150080

出　　处：《黑龙江大学自然科学学报》2024年第3期302-309,共8页Journal of Natural Science of Heilongjiang University

基　　金：西藏自治区科技计划项目(XZ201801-GA-15)。

摘　　要：建立一种测定黑木耳黑色素含量的系统方法——H_(2)O_(2)氧化-荧光法,并测定了黑木耳黑色素含量。筛选了黑木耳黑色素氧化后产生的荧光的测定条件,并对检出限、精密度、稳定性和加标回收实验进行了评价。黑木耳黑色素的最佳激发波长为340 nm,发射波长为460 nm。当NaOH浓度为0.15 mol·L^(-1)、H_(2)O_(2)浓度为10%、氧化温度为60℃、氧化最佳时间为1.5 h、超声时间为40 min时,黑色素浓度在200~1200μg·mL^(-1)与荧光强度呈良好的线性关系,线性回归方程的R^(2)=0.9960,检出限为0.92μg·mL^(-1),平均回收率为102.75%,稳定性和精密度分别为1.19%和1.29%,黑木耳中黑色素含量为2.10%。以上结果表明,H_(2)O_(2)氧化-荧光法可以准确、简便地定量黑木耳中的黑色素。A method for the determination of melanin in Auricularia heimuer by H_(2)O_(2) oxidation fluorescence analysis was established and the content of melanin in Auricularia auricula was determined.The conditions for the determination of fluorescence produced by the oxidation of melanin in Auricularia auricula were screened and the detection limit,precision,stability and standard addition recovery experiments were evaluated.The melanin in Auricularia auricula has an optimal excitation wavelength of 340 nm and an optimal emission wavelength of 460 nm.When the concentration of NaOH is 0.15 mol L^(-1),the concentration of H_(2)O_(2) is 10%,the oxidation temperature is 60℃,the oxidation time is 1.5 h,and the ultrasonic time is 40 min,the concentration of melanin in the range of 200~1200μg·mL^(-1)shows a good linear correlation with the fluorescence intensity,and R^(2)of the linear regression equation is 0.9960,the limit of detection is 0.92μg·mL^(-1),and the average recovery is 102.75%.The stability and precision are 1.19%and 1.29%,respectively,and the content of melanin in Auricularia auricula is 2.10%.The above results indicate that the H_(2)O_(2) oxidation-fluorescence method can quantify the melanin in Auricularia auricula accurately and conveniently.

关 键 词：黑木耳 黑色素 H_(2)O_(2)氧化 荧光分析法 

分 类 号：O657.3[理学—分析化学]