Phosphorylation of ZmAL14 by ZmSnRK2.2 regulates drought resistance through derepressing ZmROP8 expression  

作　　者：Yalin Wang Jinkui Cheng Yazhen Guo Zhen Li Shuhua Yang Yu Wang Zhizhong Gong 

机构地区：[1]State Key Laboratory of Plant Environmental Resilience,Frontiers Science Center for Molecular Design Breeding,College of Biological Sciences,China Agricultural University,Beijing 100193,China [2]Center for Crop Functional Genomics and Molecular Breeding,China Agricultural University,Beijing 100193,China [3]College of Life Sciences,Institute of Life Science and Green Development,Hebei University,Baoding 071002,China

出　　处：《Journal of Integrative Plant Biology》2024年第7期1334-1350,共17页植物学报（英文版）

基　　金：supported by National Key Research and Development Program of China(2022YFF1001600);STI2030-Major Projects(2030ZD0407101).

摘　　要：Drought stress has negative effects on crop growth and production.Characterization of transcription factors that regulate the expression of drought-responsive genes is critical for understanding the transcriptional regulatory networks in response to drought,which facilitates the improvement of crop drought tolerance.Here,we identified an Alfin-like(AL)family gene ZmAL14 that negatively regulates drought resistance.Overexpression of ZmAL14 exhibits susceptibility to drought while mutation of ZmAL14 enhances drought resistance.An abscisic acid(ABA)-activated protein kinase ZmSnRK2.2 interacts and phosphorylates ZmAL14 at T38 residue.Knockout of ZmSnRK2.2 gene decreases drought resistance of maize.A dehydration-induced Rho-like small guanosine triphosphatase gene ZmROP8 is directly targeted and repressed by ZmAL14.Phosphorylation of ZmAL14 by ZmSnRK2.2 prevents its binding to the ZmROP8 promoter,thereby releasing the repression of ZmROP8 transcription.Overexpression of ZmROP8 stimulates peroxidase activity and reduces hydrogen peroxide accumulation after drought treatment.Collectively,our study indicates that ZmAL14 is a negative regulator of drought resistance,which can be phosphorylated by ZmSnRK2.2 through the ABA signaling pathway,thus preventing its suppression on ZmROP8 transcription during drought stress response.

关 键 词：ZmAL14 drought tolerance transcription factor MAIZE 

分 类 号：S332.4[农业科学—作物遗传育种]