A vicinal oxygen chelate protein facilitates viral infection by triggering the unfolded protein response in Nicotiana benthamiana  

作　　者：Zhihong Guo Ning Jiang Menglin Li Hongfang Guo Qi Liu Xinyu Qin Zongying Zhang Chenggui Han Ying Wang 

机构地区：[1]Ministry of Agriculture and Rural Affairs Key Laboratory of Pest Monitoring and Green Management,College of Plant Protection,China Agricultural University,Beijing 100193,China

出　　处：《Journal of Integrative Plant Biology》2024年第7期1481-1499,共19页植物学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(32270165 and 31872921);in part by China Agricultural Industry Technology System(Grant No.CARS-170304).

摘　　要：Vicinal oxygen chelate(VOC)proteins are members of an enzyme superfamily with dioxygenase or non-dioxygenase activities.However,the biological functions of VOC proteins in plants are poorly understood.Here,we show that a VOC in Nicotiana benthamiana(NbVOC1)facilitates viral infection.NbVOC1 was significantly induced by infection by beet necrotic yellow vein virus(BNYVV).Transient overexpression of NbVOC1 or its homolog from Beta vulgaris(BvVOC1)enhanced BNYVV infection in N.benthamiana,which required the nuclear localization of VOC1.Consistent with this result,overexpressing NbVOC1 facilitated BNYVV infection,whereas,knockdown and knockout of NbVOC1 inhibited BNYVV infection in transgenic N.benthamiana plants.NbVOC1 interacts with the basic leucine zipper transcription factors bZIP17/28,which enhances their self-interaction and DNA binding to the promoters of unfolded protein response(UPR)-related genes.We propose that bZIP17/28 directly binds to the NbVOC1 promoter and induces its transcription,forming a positive feedback loop to induce the UPR and facilitating BNYVV infection.Collectively,our results demonstrate that NbVOC1 positively regulates the UPR that enhances viral infection in plants.

关 键 词：beet necrotic yellow vein virus bZIP17/28 Nicotiana benthamiana unfolded protein response VOC1 

分 类 号：S432.41[农业科学—植物病理学]