Sporophytic control of tapetal development and pollen fertility by a mitogen-activated protein kinase cascade in rice  

作　　者：Jianguo Zeng Manman Duan Yiqing Wang Guangtao Li Yujing You Jie Shi Changhao Liu Jinyang Zhang Juan Xu Shuqun Zhang Jing Zhao 

机构地区：[1]Department of Plant Pathology,Nanjing Agricultural University,Nanjing 210095,China [2]State Key Laboratory of Plant Physiology and Biochemistry,College of Life Sciences,Zhejiang University,Hangzhou 310058,China [3]Division of Biochemistry,University of Missouri,Columbia,65211MO,USA

出　　处：《Journal of Integrative Plant Biology》2024年第7期1500-1516,共17页植物学报（英文版）

基　　金：supported by grants from the National Natural Science Foundation of China(Young Scientist Fund,YSF)(32100288);Natural Science Foundation for Youths of Jiangsu Province(BK20200558);Fundamental Research Funds for the Central Universities(ZJ22195005).

摘　　要：Tapetum,the innermost layer of the anther wall,provides essential nutrients and materials for pollen development.Timely degradation of anther tapetal cells is a prerequisite for normal pollen development in flowering plants.Tapetal cells facilitate male gametogenesis by providing cellular contents after highly coordinated programmed cell death(PCD).Tapetal development is regulated by a transcriptional network.However,the signaling pathway(s)involved in this process are poorly understood.In this study,we report that a mitogen-activated protein kinase(MAPK)cascade composed of OsYDA1/OsYDA2-OsMKK4-OsMPK6 plays an important role in tapetal development and male gametophyte fertility.Loss of function of this MAPK cascade leads to anther indehiscence,enlarged tapetum,and aborted pollen grains.Tapetal cells in osmkk4 and osmpk6 mutants exhibit an increased presence of lipid body-like structures within the cytoplasm,which is accompanied by a delayed occurrence of PCD.Expression of a constitutively active version of OsMPK6(CA-OsMPK6)can rescue the pollen defects in osmkk4 mutants,confirming that OsMPK6 functions downstream of OsMKK4 in this pathway.Genetic crosses also demonstrated that the MAPK cascade sporophyticly regulates pollen development.Our study reveals a novel function of rice MAPK cascade in plant male reproductive biology.

关 键 词：DEGRADATION MAPK cascade pollen fertility RICE TAPETUM 

分 类 号：S511[农业科学—作物学]