MfCdtf1基因对桃褐腐病菌生长、产孢和致病力的影响  

MfCdtf1 gene affects mycelial growth,sporulation and pathogenicity of peach brown rot fungus Monilinia fructicola

作　　者：杨静雅 曾哲政 肖媛玲 蔡民政 吴沛珊 魏闻恺 阴伟晓 罗朝喜[1,2] Yang Jingya;Zeng Zhezheng;Xiao Yuanling;Cai Minzheng;Wu Peishan;Wei Wenkai;Yin Weixiao;Luo Chaoxi(National Key Laboratory for Germplasm Innovation&Utilization of Horticultural Crops,Huazhong Agricultural University,Wuhan 430070,Hubei Province,China;Hubei Key Laboratory of Plant Pathology,College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070,Hubei Province,China)

机构地区：[1]华中农业大学,果蔬园艺作物种质创新与利用全国重点实验室,武汉430070 [2]华中农业大学植物科技学院,湖北省作物病害监测及安全控制重点实验室,武汉430070

出　　处：《植物保护学报》2024年第3期672-683,共12页Journal of Plant Protection

基　　金：国家现代农业(桃)产业技术体系(CARS-30);国家自然科学基金(31371896)。

摘　　要：为探究桃褐腐病菌实生链核盘菌Monilinia fructicola环磷酸腺苷依赖性转录因子(cyclic adenosine monophosphate dependent transcription factor,Cdtf)在侵染植物寄主过程中发挥的作用,以野生型菌株Bmpc7为研究对象,通过基因组学和侵染早期转录组学分析预测Cdtf1基因的结构及功能,对该基因进行敲除并对敲除转化子和回补转化子进行表型和抗逆性测定。结果显示,MfCdtf1在桃褐腐病菌侵染早期上调表达明显,接种后1 h和24 h时MfCdtf1的表达量显著上升,表明MfCdtf1参与调控桃褐腐病菌的侵染和致病。对3个纯合MfCdtf1敲除转化子和3个杂合回补转化子的表型进行观察,发现MfCdtf1基因的缺失使桃褐腐病菌的菌丝生长速率和致病力显著降低,产孢能力和色素合成能力丧失。甘油、H_(2)O_(2)、十六烷基三甲基溴化铵、十二烷基苯磺酸钠、CaCl_(2)、KCl和NaCl等多种外源胁迫对敲除转化子ΔMfCdtf1的生长抑制率显著低于对野生型菌株Bmpc7的生长抑制率,表明MfCdtf1的缺失降低了桃褐腐病菌对外源胁迫的敏感性。To investigate the role of the cyclic adenosine monophosphate-dependent transcription factor gene Cdtf1 in the pathogenesis of the peach brown rot fungus Monilinia fructicola,the wild-type strain Bmpc7 was used to predict the structure and function of MfCdtf1 gene based on genomic and early transcriptomic analyses.The target gene was knocked out and subsequently complemented,and phenotype and stress resistance of transformants were measured.The results showed that MfCdtf1 expression varied significantly during the early infection stage of M.fructicola.MfCdtf1 expression increased significantly at 1 h and 24 h after inoculation,suggesting that it was involved in regulation of M.fructicola infection on peach fruit.Phenotypic observation of three homozygous MfCdtf1 knockout transformants and three heterozygous complemented transformants showed that the knockout of MfCdtf1 gene resulted in reduced mycelial growth rate and virulence of M.fructicola,lost sporulation and pigment synthesis abilities.The test of stresses such as glycerol,H_2O_2,hexadecyl trimethyl ammonium bromide,sodium dodecyl sulfate,CaCl_2,KCl and NaCl showed that the growth rate ofΔMfCdtf1 strains was lower than that of wild-type strain Bmpc7,indicating that the absence of MfCdtf1 reduced the sensitivity to exogenous stresses.

关 键 词：桃褐腐病 实生链核盘菌 MfCdtf1基因 致病机制 外源胁迫 抗逆性 

分 类 号：S436.621.13[农业科学—农业昆虫与害虫防治]