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作 者:张静 郭静 孔令伟 杨柳 郑燕 陶波[1] Zhang Jing;Guo Jing;Kong Lingwei;Yang Liu;Zheng Yan;Tao Bo(College of Plant Protection,Northeast Agricultural University,Harbin 150030,Heilongjiang Province,China)
机构地区:[1]东北农业大学植物保护学院,哈尔滨150030
出 处:《植物保护学报》2024年第3期731-740,共10页Journal of Plant Protection
基 金:国家重点研发计划(2016YFD0200500)。
摘 要:为解决除草剂防除困难的问题,采用室内盆栽试验测定了PX、PM和Mn2+三种代谢酶抑制剂对精喹禾灵、莠去津、烟嘧磺隆、硝磺草酮和烯禾啶防除稗草Echinochloa crus-galli的增效作用,测定不同用量代谢酶抑制剂PX对这5种除草剂的增效作用及其与莠去津混用后稗草体内谷胱甘肽转移酶(glutathione S-transferase,GST)和细胞色素P450(cytochrome P450,CYP450)活性的变化。结果显示,在3种代谢酶抑制剂中,代谢酶抑制剂PX对精喹禾灵、莠去津、烟嘧磺隆、硝磺草酮和烯禾啶5种除草剂的增效作用最显著。不同用量代谢酶抑制剂PX对不同除草剂的增效作用不同,其中75 g(a.i.)/hm^(2)代谢酶抑制剂PX对莠去津的增效最佳,株防除效果和鲜重防除效果分别增加了35.33个百分点和37.33个百分点。莠去津与代谢酶抑制剂PX混用后,稗草体内GST和CYP450活性均较对照显著降低。表明除草剂与代谢酶抑制剂PX混用能增加除草剂对稗草的防除效果,可以达到减施除草剂的目的。To solve the problem of herbicide control,an indoor pot test was conducted to investigate the effect of three metabolic enzyme inhibitors on the control of barnyardgrass by quinquefolium,atrazine,nicosulfuron,nitroxsulfuron and allylhydrazine,and in particular,to determine the potentiating effect of the metabolism enzyme inhibitor,PX,on the five herbicides at different concentrations,and changes in the activities of glutathione S-transferase(GST)and cytochrome P450(CYP450)of the barnyardgrass after mixing with atrazine.The results showed that among the three metabolic enzyme inhibitors,PX had the most significant synergistic effect on five herbicides,namely,quinquefolinate,atrazine,nicosulfuron,nitroxsulfuron and alloxan.Different concentrations of metabolic enzyme inhibitor PX had different synergistic effects on different herbicides,among which 75 g(a.i.)/hm~2 of metabolic enzyme inhibitor PX was most effective against atrazine,with an increase of 35.33 and 37.33 percentage points in plant control and fresh weight control,respectively.After mixing atrazine with metabolic enzyme inhibitor PX,the GST and CYP450 activities of barnyard grass were significantly reduced compared with the control.It shows that the mixing of herbicide and metabolic enzyme inhibitor PX can increase the effect of herbicide on barnyardgrass,and can achieve the purpose of reducing the application of herbicides.
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