苹果病毒检测技术  

Detection Methods of Apple Tree Viruses

作　　者：刘成龙 范旭东[1] 任芳[1] 张尊平[1] 胡国君[1] 董雅凤[1] LIU Chenglong;FAN Xudong;REN Fang;ZHANG Zunping;HU Guojun;DONG Yafeng(National Center for Eliminating Viruses from Deciduous Fruit Tree,Institute of Pomology of Chinese Academy of Agricultural Sciences,Xingcheng,Liaoning 125100,China)

机构地区：[1]中国农业科学院果树研究所国家落叶果树脱毒中心,辽宁兴城125100

出　　处：《园艺学报》2024年第7期1580-1594,共15页Acta Horticulturae Sinica

基　　金：辽宁省科学技术计划项目(2022JH2/101300189);山东省重点研发计划项目(2022LZGCQY008);中国农业科学院科技创新工程项目(CAAS-ASTIP-2016-RIP-05)。

摘　　要：简述了苹果病毒检测的指示植物检测法、电子显微镜检测技术、血清学检测技术、核酸杂交检测技术、PCR检测技术、逆转录重组酶聚合酶扩增检测技术、逆转录环介导恒温扩增检测技术、基因芯片检测技术、高通量测序检测技术的原理和特点,综述了不同检测技术取得的进展,展望了今后研究的主要方向。In this paper,the principle and characteristics of detection techniques for apple tree virus were briefly summarized,including indicator plant method,transmission electron microscopy,serological detection,nucleic acid hybridization technique,reverse transcriptase polymerase amplification technique,reverse transcription recombinase polymerase amplification technique,reverse transcription-loop mediated thermostatic amplification technique,gene microarray and high-throughput sequencing technique.The progress of different technologies for apple tree viruses detection were reviewed.In addition,future research directions are prospected.

关 键 词：苹果 病毒 检测 研究进展 

分 类 号：S661.1[农业科学—果树学]