Root-derived long-distance signals trigger ABA synthesis and enhance drought resistance in Arabidopsis  

作　　者：Qian-Qian Liu Jin-Qiu Xia Jie Wu Yi Han Gui-Quan Zhang Ping-Xia Zhao Cheng-Bin Xiang 

机构地区：[1]Division of Life Sciences and Medicine,Division of Molecular&Cell Biophysics,Hefei National Science Center for Interdisciplinary Sciences at the Microscale,University of Science and Technology of China,The Innovation Academy of Seed Design,Chinese Academy of Sciences,Hefei,Anhui 230027,China [2]College of Life Sciences,Anhui Agricultural University,Hefei,Anhui 230036,China [3]College of Agronomy,South China Agricultural University,Guangzhou,Guangdong 510642,China [4]School of Biology and Basic Medical Sciences,Soochow University,Suzhou,Jiangsu 215123,China

出　　处：《Journal of Genetics and Genomics》2024年第7期749-761,共13页遗传学报（英文版）

基　　金：supported by grants from the National Natural Science Foundation of China(31900230 to P.X.Z.);the China Postdoctoral Science Foundation(2020T130634 and 2019M652200 to P.X.Z.).

摘　　要：Vascular plants have evolved intricate long-distance signaling mechanisms to cope with environmental stress,with reactive oxygen species(ROS)emerging as pivotal systemic signals in plant stress responses.However,the exact role of ROS as root-to-shoot signals in the drought response has not been determined.In this study,we reveal that compared with wild-type plants,ferric reductase defective 3(frd3)mutants exhibit enhanced drought resistance concomitant with elevated NINE-CIS-EPOXYCAROTENOID DIOXYGENASE 3(NCED3)transcript levels and abscisic acid(ABA)contents in leaves as well as increased hydrogen peroxide(H_(2)O_(2))levels in roots and leaves.Grafting experiments distinctly illustrate that drought resistance can be conferred by the frd3 rootstock regardless of the scion genotype,indicating that long-distance signals originating from frd3 roots promote an increase in ABA levels in leaves.Intriguingly,the drought resistance conferred by the frd3 mutant rootstock is weakened by the CAT2-overexpressing scion,suggesting that H_(2)O_(2)may be involved in long-distance signaling.Moreover,the results of comparative transcriptome and proteome analyses support the drought resistance phenotype of the frd3 mutant.Taken together,our findings substantiate the notion that frd3 root-derived long-distance signals trigger ABA synthesis in leaves and enhance drought resistance,providing new evidence for root-to-shoot long-distance signaling in the drought response of plants.

关 键 词：frd3 Drought resistance Root-to-shoot long-distance signals Reactive oxygen species(ROS) H_(2)O_(2) Iron ABA 

分 类 号：Q945[生物学—植物学]