Genome mining of multi-substituted alkylresorcinols from a hybrid highly reducing-and typeⅢ-polyketide pathway  

作　　者：Huaran Zhang Yuting Huang Yingjie Tang Dekun Kong Yi Zou 

机构地区：[1]College of Pharmaceutical Sciences,Southwest University,Chongqing 400715,China

出　　处：《Chinese Chemical Letters》2024年第7期345-349,共5页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.22277100);the National Key R&D Program of China(No.2020YFA0907700);the Fundamental Research Funds for the Central Universities(Nos.SWU-KQ22034 and SWUXDPY22009);the Science and Technology Innovation Key R&D Program of Chongqing(No.CSTB2022TIAD-STX0015);the Chongqing Science Funds for Distinguished Young Scientists(No.cstc2020jcyj-jqX0005)。

摘　　要：Fungal alkylresorcinols are a class of polyketides,which are commonly synthesized by the hybridization of highly reducing polyketide synthase(hrPKS)with non-reducing polyketide synthase(nrPKS).In this study,we identified and demonstrated a new assembly model for synthesizing alkylresorcinol(scirpilin A,1),which was accomplished by collaboration of a hrPKS(FscA)and a typeⅢPKS(FscB).Furthermore,three post-tailoring enzymes(FscC,FscD,and FscE)act iteratively on 1 skeleton,including successive 14e-oxidation of inert carbons,di-halogenation,and di-methylation,to form highly oxidized and multisubstituted alkylresorcinols.Our work presents an unusual synthesis manner of alkylresorcinols,sheds light on the collaborative mechanism between hrPKS and typeⅢPKS and provides three valuable enzymatic catalysts for the tailoring of alkylresorcinol family natural products in future.

关 键 词：Genome mining Alkylresorcinol hrPKS TypeⅢPKS Iterative catalysis 

分 类 号：TQ243.1[化学工程—有机化工] TQ426.97