Regulable toehold lock for the effective control of strand displacement reaction sequence and circuit leakage  

作　　者：Kuangdi Luo Yang Qin Xuehao Zhang Hanxu Ji Heao Zhang Jiangtian Li Xianjin Xiao Xinyu Wang 

机构地区：[1]Department of Breast Surgery,Second Hospital of Jilin University,Changchun 130041,China [2]Institute of Reproductive Health,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China [3]Department of Laboratory Medicine,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China [4]Department of Pancreatic Surgery,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China [5]School of Life Science and Technology,Wuhan Polytechnic University,Wuhan 430023,China

出　　处：《Chinese Chemical Letters》2024年第7期372-376,共5页中国化学快报（英文版）

基　　金：the financial support from the National Key Research and Development Program of China(No.2021YFC2701402);the Open Research Fund of State Key Laboratory of Bioelectronics,Southeast University(No.Sklb2021-k06);the Open Foundation of NHC Key Laboratory of Birth Defect for Research and Prevention(Hunan Provincial Maternal and Child Health Care Hospital)(No.KF2020007);Hunan Province Assisted Reproduction and Regenerative Medicine Clinical Demonstration Center Funded Project(No.2020SK4019);the Open Foundation of Translational Medicine National Science and Technology Infrastructure(Shanghai)(No.TMSK-2021-141)。

摘　　要：Strand displacement reaction enables the construction of enzyme-free DNA reaction networks,thus has been widely applied to DNA circuit and nanotechnology.It has the characteristics of high efficiency,universality and regulatability.However,the existing regulation tools cannot enable effective control of the reaction sequence,which undoubtedly limits the construction of complex nucleic acid circuits.Herein,we developed a regulation tool,toehold lock,and achieved strict control of reaction sequence without loss of the main reaction signal output.Furthermore,we applied the tool to scenarios such as seesaw circuits,AND/OR logic gates,and entropy-driven circuits,and respectively demonstrated its significant superiority compared to the original method.We believe that the proposed toehold lock has greatly optimized the efficiency of DNA strand displacement-based networks,and we anticipate that the tool will be widely used in multiple fields.

关 键 词：DNA circuit Toehold lock DNA strand displacement Reaction sequence Seesaw circuit 

分 类 号：Q503[生物学—生物化学]