不同基因型长穗偃麦草幼胚诱导再生体系的构建和比较研究  

Construction and Comparison of Induction and Regeneration System for Immature Embryos of Different Genotypes of Elytrigia elongata

作　　者：张然 刘岳含 王思宁 董笛 刘亚玲 毛培春[4] 邹博坤 李晓霞 ZHANG Ran;LIU Yuehan;WANG Sining;DONG Di;LIU Yaling;MAO Peichun;ZOU Bokun;LI Xiaoxia(Institute of Ecological Protection and Restoration,Chinese Academy of Forestry/Grassland Research Center,National Forestry and Grassland Administration,Beijing 100091,China;Xinjiang Uygur Autonomous Region Grassland Station,Urumqi 830000,China;National Center of Pratacultural Technology Innovation(under preparation)/Inner Mongolia Grassland Technology Innovation Center Co.,Ltd,Hohhot 010070,China;Institute of Grassland,Flowers and Ecology,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China)

机构地区：[1]中国林业科学研究院生态保护与修复研究所/国家林业和草原局草原研究中心,北京100091 [2]新疆维吾尔自治区草原总站,新疆乌鲁木齐830000 [3]国家草业技术创新中心(筹)/内蒙古草业技术创新中心有限公司,内蒙古呼和浩特010070 [4]北京市农林科学院草业花卉与景观生态研究所,北京100097

出　　处：《中国草地学报》2024年第7期11-17,共7页Chinese Journal of Grassland

基　　金：2023年国家草业技术创新中心(筹)重大创新平台建设专项(CCPTZX2023B01);中国林业科学研究院“揭榜挂帅”配套专项(CAFYBB2022XA002)。

摘　　要：长穗偃麦草是一种耐盐碱、高产优质的多年生牧草和生态草,在我国盐碱地改良和滨海地区生态环境建设中占有重要地位,但其生物技术体系尚不成熟。本研究以来源不同的8个长穗偃麦草种质为试验材料,利用组织培养技术开展幼胚再生体系构建,筛选适宜构建遗传转化体系的优异受体材料。结果表明,在改良后的诱导培养基(MS培养基+0.5 g/L L-谷氨酸+0.5 g/L L-脯氨酸+0.3 g/L酶水解酪蛋白+30 g/L麦芽糖+2 mg/L 2,4-D+7 g/L琼脂)上,长穗偃麦草种质培养第3 d形成较小的愈伤组织,培养4周后分化。不同基因型长穗偃麦草在相同培养基上的幼胚愈伤组织诱导率、分化率和再生时间差异显著,其中4号材料的幼胚出愈率高达97.92%,分化率为88.33%,生根率为100.00%,再生时间较短,为56.2 d,可作为长穗偃麦草遗传转化的理想底盘材料。Elytrigia elongata is a kind of perennial forage and ecological grass with saline-alkali toler⁃ance,high yield and good quality,which plays an important role in the improvement of saline-alkali land and ecological environment construction in coastal areas in China.However,its biotechnology system is still immature.In this study,8 germplasm from different sources of Elytrigia elongata were used as experi⁃mental materials,tissue culture technology was used to construct the young embryo regeneration system,and excellent acceptor materials suitable for constructing genetic transformation system were screened.The results showed that on the modified induction medium(MS medium+0.5 g/L L-glutamic acid+0.5 g/L Lproline+0.3 g/L enzymatically hydrolyzed casein+30 g/L maltose+2 mg/L 2,4-D+7 g/L AGAR),a smaller callus was formed on the third day of Elytrigia elongata germplasm culture,and differentiation occurred after 4 weeks of culture.The callus induction rate,differentiation rate,and regeneration time of young embryo of different genotypes were significantly different on the same medium.Among them,the callus induction rate of young embryos of germplasm No.4 was as high as 97.92%,the differentiation rate was 88.33%,the rooting rate was 100.00%,and the regeneration time was relatively short(56.2 d),which could be used as an ideal chassis material for genetic transformation of Elytrigia elongata.

关 键 词：长穗偃麦草 愈伤组织 幼胚 再生体系 

分 类 号：S543.9[农业科学—作物学]