蠋蝽卵巢发育动态及其与光周期的关系  

Developmental dynamics of the ovary of Arma chinensis and its relationship with photoperiod

作　　者：元晓艳 李宏光 王玉生 伍绍龙 邓玉莲 李靖 赵泽影 肖艳松 谭琳[1] YUAN Xiaoyan;LI Hongguang;WANG Yusheng;WU Shaolong;DENG Yulian;LI Jing;ZHAO Zeying;XIAO Yansong;TAN Lin(College of Plant Protection,Hunan Agricultural University,Changsha 410128,China;Chenzhou Branch of Hunan Tobacco Company,Chenzhou 423000,China;Hunan Tobacco Company,Changsha 410004,China)

机构地区：[1]湖南农业大学植物保护学院,长沙410128 [2]湖南省烟草公司郴州市公司,郴州423000 [3]湖南省烟草公司,长沙410004

出　　处：《植物保护》2024年第4期118-125,153,共9页Plant Protection

基　　金：湖南省烟草公司郴州市公司项目(CZYC2021JS06,CZYC2022JS03);湖南省教育厅科学研究优秀青年项目(22B0216)。

摘　　要：蠋蝽Arma chinensis是一种重要的商品化天敌昆虫,而卵巢发育动态及其调控机制的研究有助于准确预测昆虫的产卵盛期和繁殖能力,对于规模化繁育蠋蝽的种群动态预测和调控具有重要意义。本文研究了蠋蝽卵巢的发育过程以及光周期对卵巢发育的影响,结果表明,蠋蝽雌成虫的内生殖系统由卵巢、侧输卵管、中输卵管、受精囊和端丝等组成。根据卵巢形态、卵黄沉积水平和卵子发生情况等典型特征,将其卵巢发育过程分为Ⅰ级(乳白透明期)、Ⅱ级(卵黄沉积期)、Ⅲ级(成熟待产期)、Ⅳ级(产卵盛期)和Ⅴ级(产卵末期)等5个级别。进一步研究发现,光周期对蠋蝽雌成虫的卵巢发育进程有显著影响,在长日照光周期L∥D=18 h∥6 h和16 h∥8 h时卵巢发育较快,卵巢长度、宽度,卵巢指数和卵子数量均显著高于其他光周期;而在光周期L∥D=14 h∥10 h、12 h∥12 h、10 h∥14 h、8 h∥16 h和6 h∥18 h下,卵巢发育明显减缓,大多处于乳白透明期。研究结果可为蠋蝽的生殖生物学研究提供科学依据,并为其规模化人工繁育和生防应用提供参考。The Arma chinensis is an important commercial natural enemy.Understanding the developmental dynamics of ovaries and their regulating mechanisms is helpful to the accurate prediction of the egg-laying period and fecundity of female adults,and may offer an insights into predicting and regulating the occurrence of A.chinensis.This study investigated the ovary development process of A.chinensis and the effect of photoperiod on ovarian development.The results revealed that the female adult reproductive system of A.chinensis was mainly composed of the ovary,lateral oviduct,median oviduct,spermatheca and terminal filament.According to the morphological characteristics of ovaries,yolk deposition status and egg maturity,progress of ovarian development was categorized into five levels:transparent period(levelⅠ),vitellogenesis period(levelⅡ),egg maturation period(levelⅢ),egg-laying period(levelⅣ),and late egg-laying period(levelⅤ).Further studies demonstrated that photoperiod significantly influenced the oogenesis of female adults.Ovaries of female adults developed optimally under long photoperiods(L∥D=18 h∥6 h and 16 h∥8 h),with maximum ovary length and width,ovarian indexes,eggs number compared to other photoperiods.Conversely,under photoperiods of L∥D=14 h∥10 h,12 h∥12 h,10 h∥14 h,8 h∥16 h and 6 h∥18 h,ovarian development was delayed,with most ovaries remaining in the transparent period(level I).This study contributes to our understanding of the reproductive biology of A.chinensis and provides a theoretical basis for its mass production and successful application in the future.

关 键 词：蠋蝽 卵巢发育 卵巢分级 光周期 

分 类 号：S476.2[农业科学—农业昆虫与害虫防治]