荔枝园分别间作距瓣豆与广东金钱草对土壤养分、微生物群落结构及多样性的影响  

Effect of Intercropping Centrosema pubescens or Grona styracifolia of Litchi Orchard on the Soil Nutrients,Microbial Community Structure and Diversity

作　　者：袁秉琛 王燕茹 孙郁婷 李承臻 虞道耿[3] YUAN Bingchen;WANG Yanru;SUN Yuting;LI Chengzhen;YU Daogeng(College of Tropical Crops,Hainan University,Haikou,Hainan 570228,China;College of Forestry,Hainan University,Haikou,Hainan 570228,China;Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101,China)

机构地区：[1]海南大学热带作物学院,海南海口570228 [2]海南大学林学院,海南海口570228 [3]中国热带农业科学院热带作物品种资源研究所,海南海口571101

出　　处：《热带作物学报》2024年第7期1411-1419,共9页Chinese Journal of Tropical Crops

基　　金：中国热带农业科学院热带作物品种资源研究所成果转移转化项目(No.PZS2022001);国家荔枝龙眼产业技术体系项目(No.CARS-32-20)。

摘　　要：为了探究荔枝园间作距瓣豆(Centrosema pubescens Benth.)、广东金钱草(Grona styracifolia)对土壤理化性质、微生物群落结构及多样性的影响,采集间作距瓣豆和广东金钱草不同模式下荔枝树行间0~40 cm深的土壤样本,测定土壤有机质、全氮、全磷、全钾、硝态氮、铵态氮、有效磷、速效钾和pH等理化因子,同时利用Illumina NovaSeq测序平台对荔枝园间作距瓣豆与广东金钱草模式下的土壤微生物进行16S扩增子检测。结果显示:0~20 cm土层的养分含量普遍高于20~40 cm土层;0~20 cm土层中,与CK相比,间作距瓣豆处理的土壤有机质含量提高7.06%,土壤全氮含量提高8.70%,土壤铵态氮含量提高103.29%;间作广东金钱草处理的土壤铵态氮含量提高69.41%,土壤硝态氮含量显著提高32.48%,土壤有效磷含量提高112.78%,土壤速效钾含量提高9.44%。20~40 cm土层中,与CK相比,间作距瓣豆处理的土壤铵态氮含量提高114.58%,土壤速效钾含量下降48.07%;间作广东金钱草处理的土壤硝态氮含量下降15.07%,土壤有效磷含量提高105.43%,土壤速效钾含量提高32.60%。在微生物多样性方面,0~20 cm土层的各项指数普遍高于20~40 cm土层;0~20 cm土层中,与CK相比,间作距瓣豆处理的ACE指数和Chao1指数分别降低7.65%和7.68%;20~40 cm土层,间作广东金钱草处理中的ACE指数和Chao1指数分别提升6.93%和6.74%。在土壤微生物群落结构方面,所有土层样品中主要的优势菌门均为酸杆菌门(Acidobacteriota)、绿弯菌门(Chloroflexi)、变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)、放线菌门(Actinobacteriota);主要的优势菌属为酸杆菌门亚群Gp2、Gp13和Bryobacter、Candidatus Solibacter、Bathyarchaeia等菌属;间作后明显增加泉古菌门(Crenarchaeota)、拟杆菌门(Bacteroidota)、疣微菌门(Verrucomicrobia)、Gp2、Gp13、Bryobacter的相对丰度。冗余分析结果表明,pH、全钾、全磷和速效钾是影响土壤微生物菌门结In order to investigate the effects of intercropping Centrosema pubescens or Grona styracifolia on soil phys-icochemical properties,microbial community structure and diversity in the litchi orchard,soil samples from 0 to 40 cm depth between litchi tree rows were collected under intercropping with either C.pubescens or G.styracifolia.Physico-chemical factors such as soil organic matter,total nitrogen,total phosphorus,total potassium,nitrate nitrogen,ammo-nium nitrogen,available phosphorus,available potassium and pH were measured.In addition,Illumina NovaSeq se-quencing platform was used for 16S amplicon sequencing to analyze the microbial communities in the soil under inter-cropping with C.pubescens or G.styracifolia in the litchi orchard.The results showed that the nutrient content in the 0-20 cm soil layer was generally higher than that in the 20-40 cm soil layer.In the 0-20 cm soil layer,the C.pubescens intercropping treatment increased soil organic matter by 7.06%,total nitrogen by 8.70%,and ammonium nitrogen by 103.29%,compared to the control.In the intercropping treatment with G.styracifolia,soil ammonium nitrogen increased by 69.41%,nitrate nitrogen increased by 32.48%,available phosphorus increased by 112.78%,and available potassium increased by 9.44%,compared to the control.In the 20-40 cm soil layer,the C.pubescens intercropping treatment in-creased soil ammonium nitrogen by 114.58%,but decreased available potassium by 48.07%,compared to the control.In the intercropping treatment with G.styracifolia,soil nitrate nitrogen decreased by 15.07%,while available phosphorus increased by 105.43%and available potassium increased by 32.60%,compared to the control.In terms of microbial diversity,the indices in the 0-20 cm soil layer were generally higher than those in the 20-40 cm soil layer.In the 0-20 cm soil layer,the ACE index and Chao1 index in the C.pubescens intercropping treatment were decreased by 7.65%and 7.68%,respectively,compared to the control.In the 20-40 cm soil layer,the ACE index and Chao1 index i

关 键 词：荔枝 间作 距瓣豆 广东金钱草 土壤养分 土壤微生物群落结构 

分 类 号：S667.1[农业科学—果树学] S551[农业科学—园艺学]