顶头孢霉菌产头孢菌素C发酵培养基优化  

Optimization in Fermentation Medium for Cephalosporin C by Cephalosporium Acremonium

作　　者：李建平 孟利钢 张世星 冯涛 于娜娜 LI Jianping;MENG Ligang;ZHANG Shixing;FENG Tao;YU Nana(Sinopharm Group Weqida Pharmaceutical Co.,Ltd.,Datong 037300,China)

机构地区：[1]国药集团威奇达药业有限公司,山西大同037300

出　　处：《化工管理》2024年第20期131-135,共5页Chemical Management

摘　　要：以头孢菌素C生产菌株顶头孢霉菌为研究对象,研究了多种氮源对头孢菌素C产量的影响。通过多因子二水平分析实验、爬坡实验和响应面法优化发酵培养基,确定最佳氮源为蛋氨酸、玉米蛋白粉、豆油,最佳发酵培养基配方:蛋氨酸为0.93 g/L、玉米蛋白粉为4.96 g/L、豆油为6.33 g/L。在此发酵配方下,头孢菌素C产量可达33.21 g/L,较初始配方提高16.24%。With the strain of recombinant cephalosporium acremonium producing cephalosporin C as the research object,the effect of multiple nitrogen sources on the production of cephalosporin C was studied.Through multi-factor two-level analysis experiment,climbing experiment and response surface method to optimize the fermentation medium,the optimal nitrogen sources were methionine,corn protein powder,and soybean oil.The optimal fermentation medium formula was:0.93 g/L of methionine,4.96 g/L of corn protein powder,and 6.33 g/L of soybean oil.Under this fermentation formula,the yield of cephalosporin C could reach 33.21 g/L,which was 16.24%higher than the original formula.

关 键 词：头孢菌素C 7-氨基头孢烷酸发酵 顶头孢霉菌 响应面法 

分 类 号：TQ465[化学工程—制药化工]