银杉愈伤组织诱导植株再生的影响因素  

Factors Influencing the Callus Induction and Plant Regeneration of Cathaya argyrophylla

作　　者：买凯乐[1] 李荣珍[1] 刘宏 覃琨 兰健花[1] 朱昌叁 冯立新[1] 苏杰南[1] 周全连[1] Mai Kaile;Li Rongzhen;Liu Hong;Qin Kun;Lan Jianhua;Zhu Changsan;Feng Lixin;Su Jienan;Zhou Quanlian(Guangxi Eco-Engineering Vocational and Technical College Guangxi Rare Indigenous Trees Propagation Center Liuzhou Key Laboratory for Conservation and Utilization of Forest Resources,Liuzhou 545004;Administration Bureau of Huaping National Nature Reserve,Guilin 541700;Administration Bureau of Dayaoshan National Nature Reserve,Jinxiu 545700;Guangxi Forestry Research Institute,Nanning 530001)

机构地区：[1]广西生态工程职业技术学院广西珍贵乡土树种良种培育中心,柳州545004 [2]广西桂林花坪国家级自然保护区管理局,桂林541700 [3]广西大瑶山国家级自然保护区管理局,金秀545700 [4]广西壮族自治区林业科学研究院,南宁530002

出　　处：《林业科学》2024年第7期56-64,共9页Scientia Silvae Sinicae

基　　金：广西青年基金项目(2017GXNSFBA198236);广西壮族自治区林业局林业科技项目(2024GXZCLK05)。

摘　　要：[目的]探索银杉成熟胚愈伤组织诱导植株再生的影响因素,为阐明影响诱导银杉愈伤组织、不定芽等的机制提供帮助。[方法]以广西金秀和广西桂林2个种源的银杉成熟胚为试验材料,利用1/2 MS、DCR、P6培养基,分别添加0.5、1.0、1.5 mg·L^(−1)的6-BA,0.2、0.4、0.6 mg·L^(−1)的NAA和0.5、1.0、1.5 mg·L^(−1)的IBA,诱导银杉愈伤组织、不定芽和不定根,得到银杉再生植株;分析银杉种源、培养基、植物生长调节剂等对银杉愈伤组织、不定芽、不定根诱导率和增殖壮芽率的影响。[结果]广西桂林种源银杉愈伤组织、不定根诱导率和增殖壮芽率最大,分别为66.48%,21.43%和55.00%。广西金秀种源银杉不定芽诱导率最大,为73.33%。适合银杉愈伤组织诱导植株再生的培养基和植物生长调节剂对于愈伤组织为DCR+0.5 mg·L^(−1)6-BA,对于不定芽为DCR+1.5 mg·L^(−1)6-BA,对于不定根为1/2 MS+0.2 mg·L^(−1)NAA+0.5 mg·L^(−1)IBA,对于增殖壮芽为DCR+1.0 mg·L^(−1)6-BA+0.4 mg·L^(−1)NAA。[结论]广西桂林种源银杉整体诱导率和增殖率相比广西金秀种源高。DCR培养基对银杉愈伤组织和不定芽诱导具有显著促进效果,1/2 MS培养基对不定根诱导具有显著促进效果。6-BA既可诱导银杉愈伤组织和又可诱导不定芽,NAA和IBA组合可诱导出银杉不定根,6-BA和NAA组合可对银杉不定芽进行增殖壮芽。[Objective]Cathaya argyrophylla is a rare and endangered plant unique to China,however it is difficult to breed.In this study,the factors influencing the induction callus of plant regeneration from mature embryo of C.argyrophylla were studied,so as to provide assistance in further elucidating the mechanisms that influence the induction of callus and adventitious buds in C.argyrophylla.[Method]The mature embryos of C.argyrophylla from two provenances in Jinxiu and Guilin,Guangxi,were used as experimental materials.The media of 1/2 MS,DCR,and P6,with adding 0.5,1.0,and 1.5 mg·L^(1)6-BA,0.2,0.4,and 0.6 mg·L^(1)NAA,and 0.5,1.0,1.5 mg·L^(1)IBA,respectively,were used to induce callus tissue,adventitious buds,and roots of C.argyrophylla,in order to obtain regenerated C.argyrophylla plants.The effects of two provenances,culture medium,plant growth regulators,etc.on the induction rate of callus,adventitious buds,adventitious roots,and proliferation rate of breeding and strengthening buds of C.argyrophylla were analyzed.[Result]The callus induction rate,adventitious root induction rate,and breeding and strengthening buds rate of C.argyrophylla from Guilin provenance were significantly higher,with values of 66.48%,21.43%,and 55.00%,respectively.The induction rate of adventitious buds in the C.argyrophylla from Jinxiu provenance was significantly higher,at 73.33%.The suitable culture medium and plant growth regulators for inducing plant regeneration from C.argyrophylla callus were:DCR+0.5 mg·L^(1)6-BA for callus induction,DCR+1.5 mg·L^(1)6-BA for adventitious bud induction,1/2 MS+0.2 mg·L^(1)NAA+0.5 mg·L^(1)IBA for adventitious root induction,and DCR+1.0 mg·L^(1)6-BA+0.4 mg·L^(1)NAA for bud proliferation and strengthening.[Conclusion]The overall induction and proliferation rates of C.argyrophylla from Guilin,Guangxi are higher than those from Jinxiu,Guangxi.DCR medium has a significant promoting effect on the induction of callus and adventitious buds in C.argyrophylla,while 1/2 MS medium has a significant promoting

关 键 词：银杉 濒危物种 成熟胚 愈伤组织 不定芽诱导 植株再生 

分 类 号：S791.19[农业科学—林木遗传育种] S722.89[农业科学—林学]