2个海拔梯度种源鬼箭锦鸡儿根际土壤细菌对温度变化的响应  

Response of rhizosphere soil bacteria to temperature changes in Caragana jubata from different elevation provenances

作　　者：张隆安 徐婷婷[3] 魏庐潞 马周娟 马飞[1,2] ZHANG Long’an;XU Tingting;WEI Lulu;MA Zhoujuan;MA Fei(School of Ecology and Environment,Ningxia University,Yinchuan 750021,Ningxia,China;Breeding Base for State Key Laboratory of Land Degradation and Ecological Restoration in Northwest China,Ningxia University,Yinchuan 750021,Ningxia,China;School of Life Science,Ningxia University,Yinchuan 750021,Ningxia,China)

机构地区：[1]宁夏大学生态环境学院,宁夏银川750021 [2]宁夏大学西北土地退化与生态恢复国家重点实验室培育基地,宁夏银川750021 [3]宁夏大学生命科学学院,宁夏银川750021

出　　处：《草业科学》2024年第7期1632-1644,共13页Pratacultural Science

基　　金：宁夏自然科学基金项目(2022AAC05008)。

摘　　要：根际土壤微生物与植物的生态适应密切相关,研究植物根际微生物多样性和群落结构对环境变化的响应有助于更好地理解植物生态适应机制。本研究以不同海拔梯度(2950和3530 m)鬼箭锦鸡儿(Caragana jubata)实生幼苗为研究对象,比较分析了其在不同温度(10和25℃)下的根系功能性状、根际土壤细菌多样性和群落结构差异。结果表明:温度变化显著影响了海拔2950 m种源鬼箭锦鸡儿根际土壤细菌的Chao1指数(P<0.05),但对海拔3530 m种源鬼箭锦鸡儿根际土壤细菌的Chao1和Shannon指数影响不显著;主坐标分析表明温度变化显著改变了海拔2950 m种源鬼箭锦鸡儿根际土壤细菌的群落结构(r=0.53,P<0.05),且与25℃温度处理相比,10℃显著增加了海拔2950 m种源的放线菌门(Actinobacteria)和假单胞菌属(Pseudomonas),以及海拔3530 m种源的Kapabacteria、纤维菌属(Cellvibrio)、Glutamicibacter、微杆菌属(Microbacterium)和诺卡菌属(Nocardia)的相对丰度(P<0.05)。同时,温度变化显著降低了海拔2950 m种源鬼箭锦鸡儿的根表面积、根尖数、根平均直径、根体积和根长(P<0.05),但是仅对海拔3530 m种源的根平均直径影响显著。Mantel检验表明,海拔2950 m种源鬼箭锦鸡儿根际土壤细菌群落结构受温度、根长和根平均直径影响显著(P<0.05),而海拔3530 m种源根际土壤菌群结构受温度、根体积、根表面积和根平均直径影响显著(P<0.05)。本研究发现,不同海拔梯度种源鬼箭锦鸡儿根际细菌多样性和群落结构以及根系性状对温度变化的响应存在差异,而且根系性状与其根际细菌的群落构建显著相关。Rhizosphere microorganisms are closely related to the ecological adaptations of plants.Studying the response of plant rhizosphere microorganisms to environmental changes can help provide a better understanding of the ecological adaptation of plants.In this study,the differences in rhizosphere soil bacterial diversity and community structure as well as the root functional traits of Caragana jubata at different elevations(A1:2950 m and A2:3530 m)were comparatively analyzed under different temperature conditions(T1:10℃and T2:25℃).The results showed that temperature change significantly affected the Chao1 index(P<0.05)of C.jubata from A1 provenance but had a weak effect on the Chao1 and Shannon indexes of C.jubata from A2 provenance.Principal coordinate analysis showed that temperature change significantly affected the rhizosphere soil community structure(r=0.53,P<0.05)of the provenance from A2.Compared with T2,T1 significantly increased the relative abundance of Actinobacteria and Pseudomonas for the provenance at A1 and increased the relative abundance of Kapabacteria,Cellvibrio,Glutamicibacter,Microbacterium,and Nocardia of C.jubata from A2 provenance(P<0.05).Meanwhile,temperature change significantly decreased the root surface area,number of root tips,mean root diameter,root volume,and root length of C.jubata from the A1 provenance(P<0.05)but only had a significant effect on the mean root diameter of C.jubata from A2 provenance.In addition,Mantel test showed that the bacterial community structure of C.jubata from A1 provenance was significantly affected by temperature,root length,and root mean diameter(P<0.05)whereas that of C.jubata from A2 provenance was significantly affected by temperature,root volume,root surface area,and root mean diameter(P<0.05).In conclusion,the present study demonstrated that the rhizosphere bacterial diversity and community and root traits of different altitude provenances of C.jubata varied in their responses to temperature changes and that root traits had a strong impact on the v

关 键 词：鬼箭锦鸡儿 海拔种源 土壤细菌 群落结构 多样性 根系性状 温度 

分 类 号：S54[农业科学—作物学]