子宫内膜癌组织中IGF2BP1mRNA,PEG10mRNA表达及与增殖基因表达的相关性和预后研究  

Expression of IGF2BP1 mRNA and PEG10 mRNA in Endometrial Carcinoma and Their correlation with Proliferation Gene Expression and Prognosis

作　　者：伍雯莹 黄娅芬 梅巧 WU Wenying;HUANG Yafen;MEI Qiao(Department of Women’s Health Care,Huangshi Maternity of Children’s Health Hospital(Affiliated Maternity and Children’s Health Hospital of Hubei Polytechnic University),Hubei Huangshi 435000,China;Department of Gynaecology,Huangshi Maternity of Children’s Health Hospital(Affiliated Maternity and Children’s Health Hospital of Hubei Polytechnic University),Hubei Huangshi 435000,China;Department of TCM Gynaecology,Huangshi Maternity of Children’s Health Hospital(Affiliated Maternity and Children’s Health Hospital of Hubei Polytechnic University),Hubei Huangshi 435000,China)

机构地区：[1]黄石市妇幼保健院(湖北理工学院附属妇幼保健院)妇女保健科,湖北黄石435000 [2]黄石市妇幼保健院(湖北理工学院附属妇幼保健院)妇科,湖北黄石435000 [3]黄石市妇幼保健院(湖北理工学院附属妇幼保健院)中医妇科,湖北黄石435000

出　　处：《现代检验医学杂志》2024年第4期16-22,共7页Journal of Modern Laboratory Medicine

基　　金：湖北省卫生健康委第三批联合基金项目(编号:WJ2019H480)。

摘　　要：目的研究子宫内膜癌(endometrial carcinoma,EC)组织中胰岛素样生长因子2 mRNA结合蛋白1(insulinlike growth factor 2 mRNA binding protein 1,IGF2BP1)mRNA,父系表达遗传印记基因10(patrilineal expression of genetic imprinting gene 10,PEG10)mRNA表达及与增殖基因表达的相关性及预后。方法选取2017年1月~2019年1月湖北理工学院附属妇幼保健院诊治的100例EC患者。实时荧光定量PCR检测EC癌组织和癌旁组织中IGF2BP1 mRNA,PEG10 mRNA及增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)mRNA,细胞周期素D1(cyclin D1)mRNA,细胞周期蛋白依赖激酶4(cyclin dependent kinase 4,CDK4)mRNA表达。免疫组织化学检测IGF2BP1,PEG10蛋白表达。相关性采用Pearson相关分析。Kaplan-Meier曲线分析不同IGF2BP1,PEG10表达组EC患者的预后差异。COX回归分析EC患者的预后影响因素。结果EC癌组织中IGF2BP1 mRNA(1.84±0.33),PEG10 mRNA(2.12±0.40),PCNA mRNA(3.14±0.42),cyclinD1 mRNA(2.81±0.36),CDK4 mRNA(2.37±0.34)高于癌旁组织(0.78±0.21,0.91±0.25,0.74±0.13,0.67±0.21,0.59±0.18),差异具有统计学意义(t=25.652~54.588,均P<0.05)。癌组织中IGF2BP1(70.00%),PEG10(72.00%)蛋白阳性率高于癌旁组织(100%,9.00%),差异具有统计学意义(χ^(2)=75.000,82.363,均P<0.05)。EC中IGF2BP1 mRNA,PEG10 mRNA表达与PCNA mRNA,cyclinD1 mRNA,CDK4 mRNA表达呈正相关(r=0.562~0.625,均P<0.05)。EC中IGF2BP1 mRNA与PEG10 mRNA表达呈显著正相关(r=0.663,P<0.05)。FIGO分期Ⅲ期、并发淋巴结转移EC癌组织中IGF2BP1(86.49%,87.50%),PEG10(89.19%,90.63%)阳性率高于FIGO分期Ⅰ~Ⅱ期(60.32%,61.90%)、无淋巴结转移(61.77%,63.24%),差异具有统计学意义(χ^(2)=6.863~8.608,均P<0.05)。IGF2BP1阳性组患者三年总体生存率70.00%(49/70)低于阴性组的90.00%(27/30);PEG10阳性组患者三年总体生存率为69.44%(50/72),低于阴性组的92.86%(26/28),差异具有统计学意义(Log-rankχ^(2)=4.133,5.491,P=0.042,0.019)。FIGO分期Ⅲ期(OR=1.449,95%CI:1.148~1.830)、并发�Objective To investigate the expression of insulin-like growth factor 2 mRNA binding protein 1(IGF2BP1)mRNA and patrilineal expression of genetic imprinting gene 10(PEG10)mRNA in endometrial carcinoma(EC)tissues and their correlation with proliferation gene expression and prognosis.Methods A total of 100 EC patients diagnosed and treated in Hubei Institute of Technology Affiliated Maternal and Child Health Hospital from January 2017 to January 2019 were selected.The expression of IGF2BP1 mRNA,PEG10 mRNA,proliferating cell nuclear antigen(PCNA),cyclin D1,and cyclin dependent kinase 4(CDK4)mRNA were examined by real-time fluorescence quantitative PCR.The expressions of IGF2BP1 and PEG10 in tissues were detected by immunochemistry,and their correlation were analyzed by Pearson correlation analysis.Kaplan-Meier curve analysis were used to analyze the prognostic differences in EC patients with different IGF2BP1 and PEG10 expression groups.COX regression was used to analyze the prognostic factors of EC patients.Results The expression levels of IGF2BP1 mRNA(1.84±0.33),PEG10 mRNA(2.12±0.40),PCNA mRNA(3.14±0.42),cyclinD1 mRNA(2.81±0.36)and CDK4 mRNA(2.37±0.34)in EC cancer tissues were higher than those in adjacent tissues(0.78±0.21,0.91±0.25,0.74±0.13,0.67±0.21,0.59±0.18),and the differences were significant(t=25.652~54.588,all P<0.05).The positive rates of IGF2BP1(70.00%)and PEG10(72.00%)in cancer tissues were higher than those in adjacent tissues(10.00%,9.00%),and the differences were statistically significant(χ^(2)=75.000,82.363,all P<0.05).The expressions of IGF2BP1 mRNA and PEG10 mRNA in EC tissues were positively correlated with PCNA mRNA,cyclinD1 mRNA,and CDK4 mRNA(r=0.562~0.625,all P<0.05).There was a significant positive correlation between IGF2BP1 mRNA and PEG10 mRNA expression in EC tissues(r=0.663,P<0.05).The positive rates of IGF2BP1(86.49%,87.50%)and PEG10(89.19%,90.63%)protein expression in EC cancer tissues with FIGO stage III and combined lymph node metastasis were higher than those in FIGO sta

关 键 词：子宫内膜癌 胰岛素样生长因子2 mRNA结合蛋白1 父系表达遗传印记基因10 增殖 临床病理特征 

分 类 号：R737.33[医药卫生—肿瘤] R730.43[医药卫生—临床医学]