子宫内膜异位症患者血清VEGFR-1和MIP-3α水平表达对术后复发的预测价值研究  

Study on the Predictive Value of Serum VEGFR-1 and MIP-3αLevel Expression in Endometriosis Patients on Postoperative Recurrence

作　　者：孙晓华 宋雨霖 李红 SUN Xiaohua;SONG Yulin;LI Hong(Maternal and Child Care Hospital of Qinhuangdao,Hebei Qinhuangdao 066000,China)

机构地区：[1]秦皇岛市妇幼保健院,河北秦皇岛066000

出　　处：《现代检验医学杂志》2024年第4期170-174,共5页Journal of Modern Laboratory Medicine

基　　金：秦皇岛市科学技术研究与发展计划任务书(编号:202301A214)。

摘　　要：目的探究血清血管内皮生长因子受体1(vascular endothelial growth factor receptor-1,VEGFR-1)和巨噬细胞炎性蛋白-3α(macrophage inflammatory protein-3α,MIP-3α)在子宫内膜异位症(endometriosis,EMs)患者中的表达以及两者联合检测对EMs患者术后复发的预测价值。方法选取2019年4月~2021年6月秦皇岛市妇幼保健院行腹腔镜手术治疗的114例EMs患者作为观察组,同期选择在该院体检的孕龄期女性114例健康体检者作为对照组。采用酶联免疫吸附法(ELISA)测定患者血清中VEGFR-1和MIP-3α水平;根据术后二年复发情况,将其分为复发组(n=78)和未复发组(n=36)。采用多因素Logistic回归分析EMs患者术后复发的影响因素;采用受试者工作特征(receiver operating characteristic,ROC)曲线分析血清VEGFR-1与MIP-3α联合检测对EMs患者术后复发的预测价值。结果与对照组相比,观察组VEGFR-1(116.25±48.57pg/ml vs 92.43±25.37pg/ml)及MIP-3α(19.25±5.24pg/ml vs 13.67±4.28pg/ml)水平升高,差异具有统计学意义(t=4.641,8.806,均P<0.05)。轻度、中度、重度EMs患者VEGFR-1水平(104.22±5.78pg/ml,118.60±6.56pg/ml,138.55±7.85pg/ml)和MIP-3α水平(15.37±1.15pg/ml,19.28±2.12pg/ml,25.42±2.56pg/ml)依次升高,差异具有统计学意义(F=147.757,133.654,均P<0.001)。复发组中后穹隆存在触痛结节及r-AFS分期(Ⅲ~Ⅳ期)占比显著大于未复发组(χ^(2)=15.139,10.310,均P<0.05);复发组术后用药6个月及以上占比显著低于未复发组(χ^(2)=15.016,P<0.001),差异具有统计学意义。多因素Logistic回归分析显示,血清VEGFR-1,MIP-3α,后穹隆存在触痛结节及r-AFS分期为EMs术后复发的危险因素(均P<0.05),术后用药6个月及以上为保护因素(P<0.05)。ROC曲线显示,血清VEGFR-1与MIP-3α联合预测EMs术后复发的曲线下面积(area under the curve,AUC)最大(0.929),其敏感度和特异度分别为85.90%和86.11%。结论VEGFR-1及MIP-3α在EMs患者血清中表达升高,且二者联合检测在预测Objective To investigate the expression of serum vascular endothelial growth factor receptor-1(VEGFR-1)and macrophage inflammatory protein-3α(MIP-3α)in patients with endometriosis(EMs)patients and the predictive value of their combined detection for postoperative recurrence in EMs patients.Methods A total of 114 patients with EMs who underwent laparoscopic surgery in Maternal and Child Care Hospital of Qinhuangdao from April 2019 to June 2021 were selected as the observation group,while 114 healthy medical checkups of women of gestational age who had their medical checkups in the hospital were selected as the control group during the same period.Enzyme-linked immunosorbent assay(ELISA)was used to determine the serum levels of VEGFR-1 and MIP-3αin the patients.According to the postoperative period of 2 years,they were divided into the recurrence group(n=78)and the non-recurrence group(n=36).Multivariate logistic regression analysis was used to analyze influencing factors of postoperative recurrence in patients with EMs.The predictive value of the combined serum VEGFR-1 and MIP-3αtest for postoperative recurrence in patients with EMs was analyzed by using the receiver operating characteristic curve(ROC)curve.Results Compared with the control group,the levels of VEGFR-1 in the observation group(116.25±48.57 pg/ml vs 92.43±25.37 pg/ml)and MIP-3α(19.25±5.24 pg/ml vs 13.67±4.28 pg/ml)were elevated,and the differences were significant(t=4.641,8.806,all P<0.05).The level of VEGFR-1(104.22±5.78 pg/ml,118.60±6.56 pg/ml,138.55±7.85 pg/ml)and the level of MIP-3α(15.37±1.15 pg/ml,19.28±2.12 pg/ml,25.42±2.56 pg/ml)in mild,moderate and severe EMs patients were increased successively,and the differences were significant(F=147.757,133.654,all P<0.001).The percentage of the presence of palpable nodules in the posterior fornix,r-AFS staging(stage III~IV)in the recurrence group was greater than those in the non-recurrence group(χ^(2)=15.139,10.310,all P<0.05),and the percentage of the recurrence group with postopera

关 键 词：血管内皮生长因子受体1 巨噬细胞炎性蛋白-3α 子宫内膜异位症 

分 类 号：R711.71[医药卫生—妇产科学] R392.11[医药卫生—临床医学]