炮制对苗药土党参有效成分含量的影响  

Effect of Different Processing Methods on the Content of Active Components of Campanμmoea javanica

作　　者：陆廷祥 徐英志[1] 罗元双[1] Lu Tingxiang;Xu Yingzhi;Luo Yuanshuang(Qiannan Medical College for Nationalities,Duyun 558000,China)

机构地区：[1]黔南民族医学高等专科学校,贵州都匀558000

出　　处：《山东化工》2024年第13期61-63,67,共4页Shandong Chemical Industry

基　　金：2019年中医药公共卫生服务补助专项“全国中药资源普查”(国中医药办科技函[2019]186号);黔南民族医学高等专科学校科研项目(QNYZ202114)。

摘　　要：目的:比较不同炮制法对苗药土党参主要成分党参炔苷及总多糖的含量影响。方法:采用高效液相色谱法(HPLC)测定土党参生品及其炮制品中党参炔苷的含量,以0.1%磷酸溶液-乙腈为流动相,梯度洗脱,检测波长为268 nm;采用紫外-可见分光光度法测定土党参生品及其炮制品中总多糖的含量,检测波长为490 nm。结果:土党参生品、蜜炙品、米炒品、麸炒品、土炒品及蒸制品中党参炔苷含量分别为(2.39±0.03),(1.92±0.05),(2.18±0.01),(2.15±0.03),(2.37±0.02),(2.17±0.03)mg/g;总多糖含量分别为(306.97±0.94),(336.73±0.93),(283.04±0.64),(286.18±0.80),(205.17±0.66),(296.51±0.97)mg/g。结论:土党参经不同方法炮制后,其有效成分相应发生变化;其中,除了蜜炙品总多糖含量高于生品外,其他炮制品中党参炔苷和总多糖含量均出现不同程度的下降趋势。Objective:To compare the effects of different processing methods of Campanμmoea javanica on the contents of main components of lobetyolin and total polysaccharide.Methods:HPLC was used to determine the contents of lobetyolin in Campanμmoea javanica processing products,using 0.1%phosphoric acid solution and acetonitrile as mobile phase,using gradient elution procedures,the detection wavelength was 268 nm.Ultraviolet-visible spectrophotometry was used to determine the content of total polysaccharide in different processing products of Campanμmoea javanica.The detection wavelength is 490 nm.Result:The lobetyolin contents of Campanμmoea javanica in raw,honey prepared,rice fried,bran fried,soil fried and steamed products were respectively(2.39±0.03),(1.92±0.05),(2.18±0.01),(2.15±0.03),(2.37±0.02),(2.17±0.03)mg/g.The total polysaccharide contents were respectively(296.51±0.94),(336.73±0.93),(283.04±0.64),(286.18±0.80),(205.17±0.66),(294.97±0.97)mg/g.Conclusion:After different processing methods,the effective components of Radix codonopsis were different,except for total polysaccharide of honey prepared products was higher than that of raw products,lobetyolin and total polysaccharide of other prepared products showed a decreasing trend to varying degrees.

关 键 词：土党参 炮制 高效液相色谱法 党参炔苷 总多糖 

分 类 号：TQ461[化学工程—制药化工] R284.1[医药卫生—中药学]