UPLC-MS同时检测尿液中多种毒品及其代谢物  

Simultaneous Detection of Multiple Drugs and Their Metabolites in Urine by UPLC-MS

作　　者：柴一麟 仇文璞 祁观 白登文 杨淑颐 杨丽 Chai Yilin;Qiu Wenpu;Qi Guan;Bai Dengwen;Yang Shuyi;Yang Li(Sichuan Provincial Drug Monitoring Technology Center(Sichuan Branch of National Drug Laboratory),Chengdu 610200,China)

机构地区：[1]四川省毒品监测技术中心(国家毒品实验室四川分中心),四川成都610200

出　　处：《山东化工》2024年第13期173-179,共7页Shandong Chemical Industry

摘　　要：建立了一种检测人体尿液中14种滥用毒品及其代谢物的内标定量法。分析仪器为AB SCIEX 6500+三重四极杆超高效液相色谱串联质谱仪,色谱柱为C_(18)色谱柱,色谱流动相为0.1%甲酸水溶液和乙腈,离子源模式为ESI正离子源,使用多反应检测模式进行准确定量。实验结果显示:14种待测物质的线性关系都非常好,最低检出限可达到0.03 ng/mL,准确度高,回收率范围是83.46%~114.60%,精密度好,日内精密度小于10%,日间精密度低于9%,而且样品经过前处理后基质效应比较小,样品经过多次冻融后还能保持稳定。该方法实际操作简单方便,定性定量结果精准,实验结果可复现,可以用于检测吸毒人员尿液中的多种毒品及其代谢物。A quantitative internal standard method has been established to detect 14 types of abused drugs and their metabolites in human urine.The analytical instrument is AB SCIEX 6500+triple quadrupole ultra-high performance liquid chromatography tandem mass spectrometer,with a C_(18)chromatographic column and a mobile phase of 0.1%formic acid aqueous solution and acetonitrile.The ion source mode is ESI positive ion source,and accurate quantification is performed using a multi reaction detection mode.The experimental results showed that the linear relationship of the 14 substances to be tested was very good,with a minimum detection limit of 0.03 ng/mL,high accuracy,and a recovery rate range of 83.46%~114.60%.The precision was good,with a daily precision of less than 10%and a daytime precision of less than 9%.Moreover,the substrate effect of the samples was relatively small after pre-treatment,and the samples remained stable after multiple freeze-thaw cycles.This method is simple and convenient to operate,with accurate qualitative and quantitative results.The experimental results can be replicated and can be used to detect various drugs and their metabolites in the urine of drug users.

关 键 词：毒品监测 尿液 超高效液相色谱—串联质谱 

分 类 号：O657.63[理学—分析化学]