急性心肌梗死患者外周血内皮细胞微粒中miR-126和P-选择素及E-选择素水平特点及其临床意义  

The levels of miR-126 and P-selectin and E-selectin in peripheral blood endothelial microparticles of acute myocardial infarction patients and their clinical significance

作　　者：马清玉 马艺萍 阿卜拉江·艾合麦提 尼格热·阿力木 帕丽达·玉山江 刘柯 穆叶赛·尼加提[3] MA Qing-yu;MA Yi-ping;ABULAJIANG Aihemati;NIGRE Alimu;PALIDA Yushanjiang;LIU Ke;MUYESAI Nijiati(Medical College,Shihezi University,Shihezi 832000,China;Graduate School of Xinjiang Medical University,Urumqi 830000,China;Emergency Center of People’s Hospital of Xinjiang Uygur Autonomous Region,Urumqi 830000,China)

机构地区：[1]石河子大学医学院,新疆维吾尔自治区石河子市832000 [2]新疆医科大学研究生院 [3]新疆维吾尔自治区人民医院急救中心

出　　处：《中国心血管病研究》2024年第7期600-605,共6页Chinese Journal of Cardiovascular Research

基　　金：国家自然科学基金地区基金项目(82060076)。

摘　　要：目的分析急性心肌梗死(AMI)患者外周血内皮细胞微粒(EMPs)中miR-126、P-选择素、E-选择素的表达水平,探究其与AMI的关系及临床意义。方法选择2021年9月至2022年9月新疆维吾尔自治区人民医院收治的45例AMI患者作为研究组,45例同期健康体检者作为对照组。冠状动脉狭窄的评估基于冠状动脉病变数量和Genisi评分。利用流式细胞仪检测外周血中的EMPs,用PCR法检测EMPs中的miR-126表达水平,通过ELISA法测定EMPs中的P-选择素、E-选择素表达。结果AMI组外周血EMPs中miR-126水平低于健康组,P-选择素和E-选择素水平显著高于健康组。外周血EMPs中miR-126水平在三支血管病变组中的表达水平明显低于双支病变组和单支病变组,且外周血EMPs中miR-126水平与Genisi评分呈负相关。外周血EMPs中P-选择素水平在三支血管病变组中的表达水平明显高于双支病变组和单支病变组,且P-选择素与Genisi评分呈正相关。多因素logistic回归显示,外周血EMPs中P-选择素、E-选择是AMI的危险因素。受试者工作曲线(ROC)分析结果显示,外周血EMPs中miR-126、P-选择素及E-选择素单独诊断AMI的效能较低,外周血EMPs中miR-126、P-选择素及E-选择素联合诊断AMI具有良好的诊断效能。结论外周血EMPs中P-选择素和E-选择素是AMI的危险因素。外周血EMPs中miR-126、P-选择素及E-选择素联合对AMI有诊断价值,可作为AMI诊断的生物学标志物。Objective To explore the expression levels of miR-126,P-selectin and E-selectin in endothelial microparticles(EMPs)from the peripheral blood of patients with acute myocardial infarction(AMI)and their relationship with AMI and their clinical significance.Methods Forty-five AMI patients admitted to the People's Hospital of Xinjiang Autonomous Region from September 2021 to September 2022 were selected as the research group,and 45 patients with healthy physical examination in the same period were selected as the control group.The assessment of coronary artery stenosis was based on the number of coronary lesions and the Genisi score.The EMPs in peripheral blood were detected using flow cytometry;the expression level of miR-126 in EMPs was detected by PCR;and the expressions of P-selectin and E-selectin in EMPs were measured by ELISA.Results The levels of miR-126 in the EMPs of the peripheral blood of the AMI group were lower than those in the healthy group,while the levels of P-selectin and E-selectin were significantly higher than those in the healthy group.The expression levels of miR-126 in EMPs of the peripheral blood in the three-vessel disease group were significantly lower than those in the two-vessel and single-vessel disease groups,and the level of miR-126 in EMPs of the peripheral blood was negatively correlated with the Genisi score.The levels of P-selectin in EMPs of the peripheral blood in the three-vessel disease group were significantly higher than those in the two-vessel and single-vessel disease groups,and P-selectin was positively correlated with the Genisi score.Multivariate logistic regression showed that the levels of P-selectin and E-selectin in peripheral blood EMPs were the risk factors for AMI.Receiver Operating Characteristic(ROC)analysis indicated that the diagnostic efficacy of miR-126,P-selectin and E-selectin in EMPs of peripheral blood for AMI was low when used individually but showed good diagnostic performance when used in combination.Conclusion P-selectin and E-selectin in peripheral

关 键 词：急性心肌梗死 内皮细胞微粒 MIR-126 P-选择素 E-选择素 

分 类 号：R542.22[医药卫生—心血管疾病]