基于流式细胞术对不同倍性草莓基因组大小的测定  

Determination of genome size of strawberry with different ploidy based on flow cytometry

作　　者：万红[1,5] 桂大萍 汪骞 袁艺[1] 杨建华[3] 阮继伟 陶磅[1] WAN Hong;GUI Daping;WANG Qian;YUAN Yi;YANG Jianhua;RUAN Jiwei;TAO Bang(Horticultural Research Institute,Yunnan Academy of Agricultural Sciences,Kunming,Yunnan,650205,China;Germplasm Bank of Wild Species,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming,Yunnan,650201,China;Lijiang Jianyi Animal Husbandry and Agriculture Development Co.Ltd.,Lijiang,Yunnan,674100,China;Flower Research Institute,Yunnan Academy of Agricultural Sciences,Kunming,Yunnan,650205,China;Yunnan Join Laboratory of Seed and Seed Industry,Kunming,Yunnan,650205,China)

机构地区：[1]云南省农业科学院园艺作物研究所,昆明650205 [2]中国科学院昆明植物研究所中国西南野生生物种质资源库,昆明650201 [3]丽江建乙农牧综合开发有限公司,云南丽江674100 [4]云南省农业科学院花卉研究所,昆明650205 [5]云南种子种业联合实验室,昆明650205

出　　处：《中国南方果树》2024年第4期164-169,共6页South China Fruits

基　　金：草莓抗枯萎病资源筛选及抗病基因挖掘(2023RCYP-03);玉溪草莓新品种绿色高效生产集成创新与产业化示范(202305AR340003-01);临沧市冬春蔬菜产业发展科技创新综合示范(202204AC100001-A06)资助。

摘　　要：以不同倍性草莓资源的嫩叶为材料,根据待测样品倍性预估基因组大小后分别选择水稻“日本晴”(基因组大小为430 Mb)、番茄(基因组大小为900 Mb)、玉米“B73”(基因组大小为2.3 Gb)作为内参,利用流式细胞仪测定样品的基因组大小。结果表明,二倍体资源,森林草莓基因组大小为(233±0.78)Mb,绿色草莓为(234±0.82)Mb;四倍体资源,西南草莓基因组大小为(492±2.12)Mb,伞房草莓(雄株)为(488±7.07)Mb,伞房草莓(雌株)为(491±4.95)Mb,伞房草莓雄株和雌株基因组大小均为首次测定;六倍体资源,麝香草莓基因组大小为(644±1.73)Mb;八倍体资源,栽培品种“章姬”基因组大小为(793±7.78)Mb,“蒙特瑞”为(761±0.00)Mb;十倍体资源,野生的择捉草莓基因组大小为(938±6.36)Mb,栽培品种“桃熏”为(1007±6.36)Mb。The young leaves of strawberry with different ploidy were used as materials.Rice variety Nipponbare(genome size of 430 Mb),tomato(genome size of 900 Mb),and maize B73(genome size of 2.3 Gb)were selected as internal references according to the estimated genome size of ploidy level of the testing samples.The genome size of the samples was determined by flow cytometry.The results showed that for diploid resources,the genome size of Fragaria vesca as(233±0.78)Mb,and the F.viridis was(234±0.82)Mb.For tetraploid resources,the genome size of F.moupinensis was(492±2.12)Mb,F.corymbosa(male plant)was(488±7.07)Mb,and the F.corymbosa(female plant)was(491±4.95)Mb,with the genome sizes of the male and female plants of the F.corymbosa being determined for the first time.For hexaploid resources,the genome size of F.moschata was(644±1.73)Mb.For octoploid resources,the cultivated variety F.ananassa Akihime had a genome size of(793±7.78)Mb,and Monterey was(761±0.00)Mb.For decaploid resources,the wild resources of F.iturupensis had a genome size of(938±6.36)Mb,while the cultivated variety Tao Xun was(1,007±6.36)Mb.

关 键 词：草莓 流式细胞术 基因组大小 

分 类 号：S668.4[农业科学—果树学]