Host-Induced Gene Silencing of Effector AGLIP1 Enhanced Resistance of Rice to Rhizoctonia solani AG1-IA  

作　　者：ZHAO Mei LIU Xiaoxue WAN Jun ZHOU Erxun SHU Canwei 

机构地区：[1]Pesticide State Key Laboratory of Green Pesticide,Guangdong Province Key Laboratory of Microbial Signals and Disease Control,College of Plant Protection,South China Agricultural University,Guangzhou 510642,China [2]College of Life Science and Engineering,Henan University of Urban Construction,Pingdingshan 467036,China

出　　处：《Rice science》2024年第4期463-474,I0031-I0034,共16页水稻科学（英文版）

基　　金：supported by the Henan Province Science and Technology Research Project, China (Grant No. 242102110232);the National Natural Science Foundation of China (Grant No. 31801677);the Major Program of Guangdong Basic and Applied Basic Research, China (Grant No. 2019B030302006)。

摘　　要：Rice sheath blight, caused by Rhizoctonia solani AG1-IA, is a major disease in rice-growing areas worldwide. Effectors of phytopathogenic fungi play important roles during the infection process of fungal pathogens onto their host plants. However, the molecular mechanisms by which R. solani effectors regulate rice immunity are not well understood. Through prediction, 78 candidate effector molecules were identified. Using the tobacco rattle virus-host induced gene silencing(TRV-HIGS) system, 45 RNAi constructs of effector genes were infiltrated into Nicotiana benthamiana leaves. The results revealed that eight of these constructs resulted in a significant reduction in necrosis caused by infection with the AG1-IA strain GD-118. Additionally, stable rice transformants carrying the double-stranded RNA construct for one of the effector genes, AGLIP1, were generated to further verify the function of this gene. The suppression of the AGLIP1 gene increased the resistance of both N. benthamiana and rice against GD-118, and also affected the growth rate of GD-118, indicating that AGLIP1 is a key pathogenic factor. Small RNA sequencing showed that the HIGS vectors were processed into si RNAs within the plants and then translocated to the fungi, leading to the silencing of the target genes. As a result, AGLIP1 might be an excellent candidate for HIGS, thereby enhancing crop resistance against the pathogen and contributing to the control of R. solani infection.

关 键 词：Rhizoctonia solani host-induced gene silencing LIPASE tobacco rattle virus 

分 类 号：S435.111.42[农业科学—农业昆虫与害虫防治]