吴屯杨PwHB7基因的克隆及生物信息学分析  

Cloning and Bioinformatics Analysis of PwHB7 Gene in Populus wutunensis

作　　者：张珂 孙婧靓 李国娇 金博众 胡语芮 邢小宇 ZHANG Ke;SUN Jingliang;LI Guojiao;JIN Bozhong;HU Yurui;XING Xiaoyu(College of Environment and Resources,Dalian Minzu University,Dalian,Liaoning 116600,China)

机构地区：[1]大连民族大学环境与资源学院,辽宁大连116000

出　　处：《天津农业科学》2024年第6期1-6,共6页Tianjin Agricultural Sciences

基　　金：2023年大连民族大学大学生创新创业训练计划省级项目(202312026188);大连民族大学学科团队建设项目(04442022121);大连民族大学人才引进项目。

摘　　要：为探究吴屯杨PwHB7基因的生物学特征及功能,以吴屯杨为材料,利用RT-PCR技术克隆吴屯杨PwHB7基因的cDNA全长序列,并通过生物信息学方法分析PwHB7全长序列的特征。结果表明:吴屯杨PwHB7基因开放阅读框(open reading frame,ORF)长度为717 bp,编码238个氨基酸。二级结构预测表明:PwHB7蛋白主要由α螺旋、无规则卷曲、延伸链、β转角组成。理化性质分析表明:该蛋白由C、H、O、N、S组成,理论等电点为4.93,脂肪指数为60.67。跨膜区分析表明:该蛋白为非跨膜蛋白。磷酸化位点分析表明:丝氨酸、苏氨酸和酪氨酸可能成为磷酸化位点。系统发育树分析表明:PwHB7基因与毛果杨PtHB7基因的亲缘关系最近。该结果为解析PwHB7基因响应干旱胁迫的分子机制奠定了基础,为吴屯杨抗旱育种提供新思路。To explore the biological characteristics and functions of the PwHB7 gene in Populus wutunensis,this study used Populus wutunensis as the material,cloned the cDNA full-length sequence of the PwHB7 gene using RT-PCR technology,and analyzed the characteristics of the PwHB7 full-length sequence through bioinformatics methods.The results showed that the open reading frame(ORF)length of the PwHB7 gene in Populus wutunensis was 717 bp,encoding 238 amino acids.Secondary structure prediction indicates that PwHB7 protein was mainly composed ofα-helix,irregular coil,extended chain,andβ-turn.Physical and chemical analysis showed that the protein was composed of C,H,O,N,and S components,with a theoretical isoelectric point of 4.93 and a fat index of 60.67.Transmembrane analysis indicated that the protein was a non transmembrane protein.Phosphorylation site analysis suggested that serine,threonine,and tyrosine mighot become phosphorylation sites.Phylogenetic tree analysis showed that the PwHB7 gene had the closest genetic relationship with Populus trichocarpa PtHB7.This result lays the foundation for analyzing the molecular mechanism of PwHB7 gene's response to drought stress and provided new ideas for drought resistant breeding of Populus wutunensis.

关 键 词：吴屯杨 PwHB7基因 克隆 序列分析 

分 类 号：S792.11[农业科学—林木遗传育种]