性别与生境因素对黑线姬鼠肠道菌群多样性的影响  

Effects of Sex and Habitat Factors on Intestinal Flora Diversity of Apodemus agrarius

作　　者：金志民[1] 黄笑然 李强 杨文建 贾修歧 JIN Zhimin;HUANG Xiaoran;LI Qiang;YANG Wenjian;JIA Xiuqi(College of Life Science and Technology,Mudanjiang Normal University,Mudanjiang,Heilongjiang 157011,China)

机构地区：[1]牡丹江师范学院生命科学与技术学院,黑龙江牡丹江157011

出　　处：《天津农业科学》2024年第6期37-43,共7页Tianjin Agricultural Sciences

基　　金：国家重点研发计划子课题(2023YFF0724603-4);黑龙江省2023年度省属高校基本科研业务费科研项目(1453QN029);牡丹江师范学院青年学术骨干项目(MNUQN202305);生物学学科卓越研究生培养模式改革创新项目(MSYZYXM2022004)。

摘　　要：为探究黑线姬鼠(Apodemus agrarius)肠道菌群的组成以及多样性,讨论性别差异和生境差异对黑线姬鼠肠道菌群的影响,采用高通量测序技术对黑线姬鼠肠道菌群的16S rRNA V3-V4区进行PCR扩增并进行分析。结果显示:农田黑线姬鼠雌雄2组样品间在门、目、属水平上排名前3的优势菌群种类大致相同,但各菌群所占比例不同;灌丛黑线姬鼠雌雄2组样品间在门、目、属水平上的前3种优势菌群不同。Alpha多样性分析表明:农田组黑线姬鼠雌雄间肠道菌群差异不显著;灌丛组黑线姬鼠雌雄间肠道菌群多样性差异显著,丰富度差异不显著;不同生境黑线姬鼠肠道菌群无显著差异。ANOSIM分析表明:农田黑线姬鼠雌雄2组样品间肠道菌群差异不显著;灌丛黑线姬鼠雌雄2组样品间肠道菌群差异极显著;2种生境黑线姬鼠组间肠道菌群差异不显著。综上所述,性别和生境对黑线姬鼠的肠道菌群的组成及多样性有影响。In order to investigate the composition and diversity of the intestinal flora of Apodemus agrarius,the effects of sex differences and habitat differences on the intestinal flora of Apodemus agrarius were discussed.The 16S rRNA V3-V4 region of the intestinal flora of Apodemus agrarius was amplified by PCR using high-throughput sequencing technology and analyzed.The results showed that the first three dominant intestinal flora were roughly the same at phylum,order and genus level between male and female groups of Apodemus agrarius in farmland,but the proportion of each bacterial group was different.The first three dominant intestinal flora were different at phylum,order and genus level between male and female groups of Apodemus agrarius in scrub.Alpha diversity analysis showed that there was no significant difference in intestinal flora between male and female of Apodemus agrarius in farmland.There was significant difference in intestinal flora diversity between male and female of Apodemus agrarius in scrub,but no significant difference in intestinal flora richness.There was no significant difference in intestinal flora of Apodemus agrarius in different habitats.ANOSIM analysis showed that there was no significant difference in intestinal flora between male and female groups of Apodemus agrarius in farmland.The intestinal flora was significantly different between male and female of Apodemus agrarius in scrub.There was no significant difference in intestinal flora between two groups of Apodemus agrarius.In conclusion,sex and habitat have influence on the composition and diversity of intestinal flora of Apodemus agrarius.

关 键 词：黑线姬鼠 肠道菌群 高通量测序 16S rRNA 

分 类 号：S764.5[农业科学—森林保护学]