叶面喷施Fe_(2)O_(3)NPs对娃娃菜NaCl胁迫的缓解效应  

Alleviation effect of foliar spraying Fe_(2)O_(3)NPs on NaCl stress of Mini Chinese Cabbage

作　　者：杨旭珍 颉建明 张婧 张雅茹 YANG Xuzhen;XIE Jianming;ZHANG Jing;ZHANG Yaru(College of Horticulture,Gansu Agricultural University,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学园艺学院,甘肃兰州730070

出　　处：《江西农业大学学报》2024年第4期856-866,共11页Acta Agriculturae Universitatis Jiangxiensis

基　　金：甘肃省科技计划资助项目(23CXNA0037、21JR7RA821)。

摘　　要：【目的】旨在探究纳米氧化铁(Fe_(2)O_(3)NPs)对娃娃菜盐胁迫的缓解效应。【方法】以“皇妃”娃娃菜为试验材料,喷施超纯水为对照,在150 mmol/L NaCl胁迫下叶面喷施不同质量浓度Fe_(2)O_(3)NPs(0,25,50,100,200,400,500 mg/L)。【结果】NaCl胁迫通过影响娃娃菜根系生长、叶细胞膜脂过氧化、抗氧化酶活性,以及AsA-GSH循环抑制植株生长。喷施不同质量浓度的Fe_(2)O_(3)NPs均能够促进娃娃菜根系生长,提高叶片谷胱甘肽还原酶(GR)、脱氢抗坏血酸还原酶(DHAR)及抗坏血酸过氧化物酶(APX)活性、抗氧化剂GSH含量及GSH/GSSG比值,降低过氧化氢(H_(2)O_(2))、超氧阴离子(O_(2)^(-))、丙二醛(MDA)、脯氨酸(Pro)含量及相对电导率(REC),有效缓解NaCl胁迫对娃娃菜幼苗生长的抑制作用。500 mg/L的Fe_(2)O_(3)NPs能够显著提高盐胁迫下娃娃菜植株总根长、根体积、根尖数以及根表面积,分别提高55.66%、56.33%、68.49%和57.61%;显著降低叶片中H_(2)O_(2)、O_(2)^(-)、MDA、Pro含量,分别降低48.77%、42.50%、23.68%、47.53%;显著增强GR、DHAR、APX活性,提高GSH含量和GSH/GSSG比值,分别增加16.39%、18.66%、56.64%和35.41%、60.26%。【结论】150 mmol/L NaCl胁迫对娃娃菜幼苗的生长具有明显的负效应,外源喷施不同质量浓度(25~500 mg/L)的Fe_(2)O_(3)NPs均能够有效缓解NaCl胁迫的抑制作用,试验条件下500 mg/L的Fe_(2)O_(3)NPs的缓解效果最优。[Objective]The aim is to explore the alleviation effect of nano-iron oxide(Fe_(2)O_(3)NPs)on salt stress of Mini Chinese Cabbage.[Method]With‘Huangfei’Mini Chinese Cabbage as the test material and ultrapure water as the control,different concentrations of Fe_(2)O_(3)NPs(0,25,50,100,200,400,500 mg/L)were sprayed on the leaves under 150 mmol/L NaCl stress.[Result]NaCl stress inhibited plant growth by affecting Mini Chinese Cabbage root growth,leaf cell membrane lipid peroxidation,antioxidant enzyme activities,and the AsA-GSH cycle.Spraying different concentrations of Fe_(2)O_(3)NPs promoted root growth,increased leaf glutathione reductase(GR),dehydroascorbate reductase(DHAR),ascorbate peroxidase(APX)activities,antioxidant GSH content and GSH/GSSG ratio,and reduced hydrogen peroxide(H_(2)O_(2)),superoxide(H_(2)O_(2)),superoxide anion(O_(2)^(-)),malondialdehyde(MDA),proline(Pro)content and relative electrical conductivity(REC).The spraying effectively mitigated the inhibitory effect of NaCl stress on the growth of Mini Chinese Cabbage seedlings.Fe_(2)O_(3)NPs at 500 mg/L significantly increased total root length,root volume,root tip number and root surface area of Mini Chinese Cabbage plants under salt stress by 55.66%,56.33%,68.49%and 57.61%,respectively;it significantly reduced the H_(2)O_(2),O_(2)^(-),MDA and Pro contents in leaves by 48.77%,42.50%,23.68%,and 47.53%,respectively;it significantly enhanced GR,DHAR,and APX activities,and increased GSH content and GSH/GSSG ratio by 16.39%,18.66%,56.64%,and 35.41%and 60.26%,respectively.[Conclusion]150 mmol/L NaCl stress had a significant negative effect on the growth of Mini Chinese Cabbage seedlings,and exogenous sprays of Fe_(2)O_(3)NPs at different concentrations(25-500 mg/L)were able to effectively alleviate the inhibitory effect of NaCl stress;the alleviating effect of 500 mg/L Fe_(2)O_(3)NPs was optimal under the experimental conditions.

关 键 词：娃娃菜 NACL胁迫 Fe_(2)O_(3)NPs 膜脂过氧化 ASA-GSH循环 

分 类 号：S634.3[农业科学—蔬菜学]