仔猪初生重对肌肉血管生成及代谢组学的影响  

Effects of Birth Weight on Muscle Angiogenesis and Metabolic Profiling in Piglets

作　　者：邹冬彬 杨运 吕仁龙 周汉林[1,3] 胡诚军[3] ZOU Dongbin;YANG Yun;LV Renlong;ZHOU Hanlin;HU Chengjun(College of Life Sciences,Hainan University,Haikou 571101,China;College of Animal Science and Technology,Huazhong Agricultural University,Wuhan 430070,China;Tropical Crop Germplasm Research Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou,571101,China)

机构地区：[1]海南大学生命科学学院,海南海口571101 [2]华中农业大学动物科学技术学院,武汉430070 [3]中国热带农业科学院热带作物品种资源研究所,海南海口571101

出　　处：《家畜生态学报》2024年第8期33-40,共8页Journal of Domestic Animal Ecology

基　　金：海南省自然科学基金青年基金项目(321QN320);中央级公益性科研院所基本科研业务费专项(1630032023009);海南省地方猪现代农业产业技术体系(HNARS-2022-2-G03)。

摘　　要：试验旨在研究仔猪初生重对肌肉血管生成的影响。将20头新生屯昌黑猪按体重分为正常初生重仔猪(1162±98 g,NBW组)和低初生重仔猪(724±31 g,LBW组),每组10头。仔猪屠宰后,收集仔猪半健肌用于血管生成及代谢物检测。结果表明:(1)与NBW仔猪相比,LBW仔猪半健肌血管生成标记物血小板-内皮细胞粘附分子1(CD31)、血管生成因子A(VEGF-A)及基质金属蛋白酶9(MMP9)的蛋白质表达水平显著降低(P<0.05)。(2)为进一步探究与肌肉血管生成相关代谢物,运用代谢组学技术筛选两组肌肉中的差异代谢物。发现NBW和LBW仔猪半健肌中差异代谢物主要富集在甘油磷脂代谢通路、不饱和脂肪酸代谢通路、精氨酸和脯氨酸代谢通路。在甘油磷脂代谢通路中,LBW组胆碱、心磷脂、2-酰-sn-甘油-3-磷酸丝氨酸及1-酰-sn-甘油-3-磷酸胆碱等代谢物显著上升(P<0.05);在不饱和脂肪酸代谢通路中,LBW组亚油酸显著下降、十八烷酸显著上升(P<0.05);在精氨酸和脯氨酸代谢通路中,LBW组S-腺苷基-L-甲硫氨酸及鸟氨酸显著下降(P<0.05)。由此可见,LBW仔猪肌肉血管生成降低可能与甘油磷脂代谢通路、不饱和脂肪酸代谢通路、精氨酸和脯氨酸代谢通路中代谢物变化有关。This study was conducted to investigate the effects of birth weight on muscle angiogenesis in piglets.Twenty newborn Tunchang black piglets were divided into two groups based on their body weight:normal birth weight piglets(NBW,1162±98 g)and low birth weight piglets(LBW,724±31 g),with 10 piglets in each group.The semitendinosus muscles of the piglets were collected for angiogenesis and metabolite determination.The results showed as follows:1)compared with NBW group,LBW group exhibited significantly decreased protein expression levels of the angiogenesis markers platelet-endothelial cell adhesion molecule 1(CD31),vascular endothelial growth factor A(VEGF-A),and matrix metalloproteinase 9(MMP9)in semitendinosus muscle(P<0.05).2)To further investigate the metabolites related to muscle angiogenesis,the metabolomics technology was employed to screen differential metabolites in the muscle of the two groups.It was found that differential metabolites in the semitendinosus muscle of NBW and LBW piglets were mainly enriched in glycerophospholipid metabolism,unsaturated fatty acid metabolism pathways,arginine and proline metabolism pathways.In the glycerophospholipid metabolism pathway,metabolites such as choline,phosphatidylserine,2-acyl-sn-glycerol-3-phosphor-serine,and 1-acyl-sn-glycerol-3-phosphate choline were significantly increased in the LBW group(P<0.05);in the unsaturated fatty acid metabolism pathway,the level of linoleic acid was significantly decreased and that of stearic acid were significantly increased in the LBW group(P<0.05);in the arginine and proline metabolism pathway,the levels of S-adenosyl-L-methionine and ornithine in muscle were significantly decreased in the LBW group(P<0.05).These results suggest that the decreased muscle angiogenesis in LBW piglets may be associated with changes in metabolites in the glycerophospholipid metabolism pathway,arginine and proline metabolism pathway,and unsaturated fatty acid metabolism pathway.

关 键 词：血管生成 半健肌 代谢组学 仔猪 低初生重 

分 类 号：S828.1[农业科学—畜牧学] S852.1[农业科学—畜牧兽医]