水苏糖对秦川牛精液冷冻保存效果的影响  

Effect of Stachyose on the Cryopreservation of Qinchuan Bull Semen

作　　者：王萌[1] 杜鑫泽 杨超群 贾鸿儒 昝林森[1] 李付强 张涛平 杨武才[1] WANG Meng;DU Xinze;YANG Chaoqun;JIA Hongru;ZAN Linsen;LI Fuqiang;ZHANG Taoping;YANG Wucai(National Beef Improvement Center,College of Animal Science and Technology,Northwest A&F University,Yangling,Shaanxi 712100;Hunan Tianhua Industrial Co.,Ltd.,Shaoyang,Hunan 417100;Shaanxi Qinbao Animal Husbandry Co.,Ltd.,Yangling,Shaanxi 712100)

机构地区：[1]西北农林科技大学动物科技学院,国家肉牛改良中心,陕西杨凌712100 [2]湖南天华实业有限公司,湖南邵阳417100 [3]陕西秦宝牧业股份有限公司,陕西杨凌712100

出　　处：《家畜生态学报》2024年第8期41-46,共6页Journal of Domestic Animal Ecology

基　　金：陕西省杨凌区2021年试验示范站(基地)科技创新与成果转化项目(TGZX2021-25);国家肉牛牦牛产业技术体系建设(CARS-37);陕西省畜禽育种“两链融合”重点专项(2022GD-TSLD-46-0104)。

摘　　要：试验主要探究在牛精液冷冻稀释液中添加水苏糖对秦川牛精液冷冻保存效果的影响。添加不同浓度(0、20、40、60、80 mg/mL)水苏糖到牛精液冷冻基础稀释液中,检测冷冻-解冻后秦川牛精子运动性能、质膜完整性、顶体完整率、线粒体活性及抗氧化性相关指标。结果表明,与对照组相比,40 mg/mL水苏糖处理显著(P<0.05)提高冷冻-解冻后精子活力(43.83%)和顶体完整率(78.70%);质膜的完整性和线粒体活性在随着水苏糖浓度的增加显著提升,但质膜的完整性在不同水苏糖浓度之间差异不显著(P>0.05);同时,40 mg/mL水苏糖组显著提高GSH-Px酶和CAT酶的活性(P<0.05),并显著降低精子细胞中的MDA含量(P<0.05)。综上表明,40 mg/mL水苏糖处理显著提升冷冻-解冻后牛精子品质和抗氧化能力,对秦川牛的冷冻精液具有较好保存效果。The experiment was to explore the effect of stachyose on semen cryopreservation of Qinchuan bull.Different concentrations of stachyose(0 mg/mL,20 mg/mL,40 mg/mL,60 mg/mL and 80 mg/ml)were supplemented to the frozen diluent of bull semen,then the sperm motility,membrane integrity,acrosome integrity,mitochondrial activity and antioxidant properties related indicators of frozen-thawed semen in Qinchuan bull were tested.The results showed that 40 mg/mL stachyose treatment significantly(P<0.05)improved sperm motility(43.83%)and acrosome integrity(78.70%)after freezing-thawing,compared with the control group;membrane integrity and mitochondrial activity were enhanced significantly with the increase of stachyose concentration(P<0.05),but the membrane integrity was not significantly different between the different concentrations of stachyose(P>0.05);in addition,the activities of GSH-Px enzyme and CAT enzyme were significantly increased(P<0.05),and the content of MDA significantly reduced(P<0.05)in the 40 mg/mL stachyose.In summary,the 40 mg/mL stachyose treatment significantly improves the quality and antioxidant capacity of freezing-thawing semen,and has a good preservation effect on the frozen semen in Qinchuan bull.

关 键 词：水苏糖 精液稀释液 冷冻保存 秦川牛 精液 

分 类 号：S823.34[农业科学—畜牧学]