基于AFS和ICP-MS测定婴幼儿配方乳粉中硒含量的关键技术探讨  

Discussion on Key Technologies for The Determination of Selenium in Infant Formula Milk Powder Based on AFS and ICP-MS

作　　者：严睿 李清清[1] YAN Rui;LI Qing-qing(Shanghai Institute of Quality Inspection and Technical Research/Key Laboratory of Milk and Dairy Products Detection and Monitoring Technology for State Market Regulation,Shanghai 200233,China)

机构地区：[1]上海市质量监督检验技术研究院/国家市场监管重点实验室(乳及乳制品检测与监控技术),上海200233

出　　处：《中国食物与营养》2024年第7期34-38,共5页Food and Nutrition in China

基　　金：上海市市场监督管理局科技项目“特殊医学用途婴儿配方粉钼、碘成分分析标准物质的研制”(项目编号:2023-37)

摘　　要：目的:探讨采用原子荧光光谱(AFS)和电感耦合等离子体质谱(ICP-MS)技术对婴幼儿配方乳粉中的硒含量进行测定,分析方法原理、检测模式以及定量方式等关键技术点对检测结果准确性以及造成结果差异的影响及原因。方法:样品经微波消解后,分别使用原子荧光光谱外标法和电感耦合等离子体质谱法的高灵敏度模式、高基体模式及标准加入法对婴幼儿配方乳粉中的硒进行测定。结果:原子荧光光谱标准曲线法和ICP-MS标准加入法定量结果与质控样品中位值比较接近,ICP-MS采用标准曲线法定量时,使用高灵敏度模式和高基体模式的测定结果均比质控中位值高。结论:当使用原子荧光光谱仪外标法和电感耦合等离子质谱仪标准加入法定量时,可获得较为可靠的结果。【Objective】To investigate the determination of selenium content in infant formula milk powder by atomic fluorescence spectrometry(AFS)and inductively coupled plasma mass spectrometry(ICP-MS),and the influence of key technical points such as analysis method principle,detection mode and quantitative method on the accuracy of detection results and the difference of results and the reasons.【Method】The samples were pre-treated with microwave digestion,and the selenium in infant formula powder was determined by high sensitivity mode,high matrix mode and standard addition method of atomic fluorescence spectrometry and inductively coupled plasma mass spectrometry,respectively.【Result】The quantitative results of the standard curve method of atomic fluorescence spectrometry and the standard addition method of ICP-MS were close to the median value of the quality control sample.When the standard curve method of ICP-MS was used,the results of the high sensitivity mode and the high matrix mode were higher than the median value of the quality control sample.【Conclusion】More reliable results can be obtained when atomic fluorescence spectrometer external standard method and inductively coupled plasma mass spectrometer standard addition method are used.

关 键 词：原子荧光光谱 电感耦合等离子体质谱 硒 婴幼儿配方乳粉 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程] O657.63[轻工技术与工程—食品科学与工程]